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Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays

Johne’s Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), results in significant economic loss to livestock production. The early detection of MAP infection in animals with extant serological assays has remained challenging due to the low sensitivity of commercially avai...

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Autores principales: Li, Lingling, Wagner, Bettina, Freer, Heather, Schilling, Megan, Bannantine, John P., Campo, Joseph J., Katani, Robab, Grohn, Yrjo T., Radzio-Basu, Jessica, Kapur, Vivek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736219/
https://www.ncbi.nlm.nih.gov/pubmed/29261761
http://dx.doi.org/10.1371/journal.pone.0189783
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author Li, Lingling
Wagner, Bettina
Freer, Heather
Schilling, Megan
Bannantine, John P.
Campo, Joseph J.
Katani, Robab
Grohn, Yrjo T.
Radzio-Basu, Jessica
Kapur, Vivek
author_facet Li, Lingling
Wagner, Bettina
Freer, Heather
Schilling, Megan
Bannantine, John P.
Campo, Joseph J.
Katani, Robab
Grohn, Yrjo T.
Radzio-Basu, Jessica
Kapur, Vivek
author_sort Li, Lingling
collection PubMed
description Johne’s Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), results in significant economic loss to livestock production. The early detection of MAP infection in animals with extant serological assays has remained challenging due to the low sensitivity of commercially available ELISA tests, a fact that has hampered the development of effective JD control programs. Our recent protein microarray-based studies identified several promising candidate antigens that are immunogenic during different stages of MAP infection. To evaluate these antigens for use in diagnostic assays and reliably identify animals with MAP infection, a multiplex (Luminex(®)) assay was developed using color-coded flourescent beads coupled to 6 MAP recombinant proteins and applied to screen 180 serum and 90 milk samples from cows at different stages of MAP infection including negative (NL), fecal test positive/ELISA negative (F+E-), and fecal positive/ELISA positive (F+E+). The results show that while serum antibody reactivities to each of the 6 antigens were highest in F+E+ group, antibody reactivity to three of the six antigens were identified in the F+E- group, suggesting that these three antigens are expressed and provoke antibody responses during the early infection stages with MAP. Further, antibodies against all six antigens were elevated in milk samples from both the F+E- and F+E+ groups in comparison to the NL group (p<0.01). Taken together, the results of our investigation suggest that multiplex bead-based assays are able to reliably identify MAP infection, even during early stages when antibody responses in animals are undetectable with widely used commercial ELISA tests.
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spelling pubmed-57362192017-12-22 Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays Li, Lingling Wagner, Bettina Freer, Heather Schilling, Megan Bannantine, John P. Campo, Joseph J. Katani, Robab Grohn, Yrjo T. Radzio-Basu, Jessica Kapur, Vivek PLoS One Research Article Johne’s Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis (MAP), results in significant economic loss to livestock production. The early detection of MAP infection in animals with extant serological assays has remained challenging due to the low sensitivity of commercially available ELISA tests, a fact that has hampered the development of effective JD control programs. Our recent protein microarray-based studies identified several promising candidate antigens that are immunogenic during different stages of MAP infection. To evaluate these antigens for use in diagnostic assays and reliably identify animals with MAP infection, a multiplex (Luminex(®)) assay was developed using color-coded flourescent beads coupled to 6 MAP recombinant proteins and applied to screen 180 serum and 90 milk samples from cows at different stages of MAP infection including negative (NL), fecal test positive/ELISA negative (F+E-), and fecal positive/ELISA positive (F+E+). The results show that while serum antibody reactivities to each of the 6 antigens were highest in F+E+ group, antibody reactivity to three of the six antigens were identified in the F+E- group, suggesting that these three antigens are expressed and provoke antibody responses during the early infection stages with MAP. Further, antibodies against all six antigens were elevated in milk samples from both the F+E- and F+E+ groups in comparison to the NL group (p<0.01). Taken together, the results of our investigation suggest that multiplex bead-based assays are able to reliably identify MAP infection, even during early stages when antibody responses in animals are undetectable with widely used commercial ELISA tests. Public Library of Science 2017-12-19 /pmc/articles/PMC5736219/ /pubmed/29261761 http://dx.doi.org/10.1371/journal.pone.0189783 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Li, Lingling
Wagner, Bettina
Freer, Heather
Schilling, Megan
Bannantine, John P.
Campo, Joseph J.
Katani, Robab
Grohn, Yrjo T.
Radzio-Basu, Jessica
Kapur, Vivek
Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title_full Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title_fullStr Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title_full_unstemmed Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title_short Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
title_sort early detection of mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736219/
https://www.ncbi.nlm.nih.gov/pubmed/29261761
http://dx.doi.org/10.1371/journal.pone.0189783
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