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Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours
The HOXB13 G84E variant is associated with risk of prostate cancer (PCa), however the role this variant plays in PCa development is unknown. This study examined 751 cases, 450 relatives and 355 controls to determine the contribution of this variant to PCa risk in Tasmania and investigated HOXB13 gen...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736598/ https://www.ncbi.nlm.nih.gov/pubmed/29259341 http://dx.doi.org/10.1038/s41598-017-18217-w |
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author | FitzGerald, Liesel M. Raspin, Kelsie Marthick, James R. Field, Matt A. Malley, Roslyn C. Thomson, Russell J. Blackburn, Nicholas B. Banks, Annette Charlesworth, Jac C. Donovan, Shaun Dickinson, Joanne L. |
author_facet | FitzGerald, Liesel M. Raspin, Kelsie Marthick, James R. Field, Matt A. Malley, Roslyn C. Thomson, Russell J. Blackburn, Nicholas B. Banks, Annette Charlesworth, Jac C. Donovan, Shaun Dickinson, Joanne L. |
author_sort | FitzGerald, Liesel M. |
collection | PubMed |
description | The HOXB13 G84E variant is associated with risk of prostate cancer (PCa), however the role this variant plays in PCa development is unknown. This study examined 751 cases, 450 relatives and 355 controls to determine the contribution of this variant to PCa risk in Tasmania and investigated HOXB13 gene and protein expression in tumours from nine G84E heterozygote variant and 13 wild-type carriers. Quantitative PCR and immunohistochemistry showed that HOXB13 gene and protein expression did not differ between tumour samples from variant and wild-type carriers. Allele-specific transcription revealed that two of seven G84E carriers transcribed both the variant and wild-type allele, while five carriers transcribed the wild-type allele. Methylation of surrounding CpG sites was lower in the variant compared to the wild-type allele, however overall methylation across the region was very low. Notably, tumour characteristics were less aggressive in the two variant carriers that transcribed the variant allele compared to the five that did not. This study has shown that HOXB13 expression does not differ between tumour tissue of G84E variant carriers and non-carriers. Intriguingly, the G84E variant allele was rarely transcribed in carriers, suggesting that HOXB13 expression may be driven by the wild-type allele in the majority of carriers. |
format | Online Article Text |
id | pubmed-5736598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57365982017-12-21 Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours FitzGerald, Liesel M. Raspin, Kelsie Marthick, James R. Field, Matt A. Malley, Roslyn C. Thomson, Russell J. Blackburn, Nicholas B. Banks, Annette Charlesworth, Jac C. Donovan, Shaun Dickinson, Joanne L. Sci Rep Article The HOXB13 G84E variant is associated with risk of prostate cancer (PCa), however the role this variant plays in PCa development is unknown. This study examined 751 cases, 450 relatives and 355 controls to determine the contribution of this variant to PCa risk in Tasmania and investigated HOXB13 gene and protein expression in tumours from nine G84E heterozygote variant and 13 wild-type carriers. Quantitative PCR and immunohistochemistry showed that HOXB13 gene and protein expression did not differ between tumour samples from variant and wild-type carriers. Allele-specific transcription revealed that two of seven G84E carriers transcribed both the variant and wild-type allele, while five carriers transcribed the wild-type allele. Methylation of surrounding CpG sites was lower in the variant compared to the wild-type allele, however overall methylation across the region was very low. Notably, tumour characteristics were less aggressive in the two variant carriers that transcribed the variant allele compared to the five that did not. This study has shown that HOXB13 expression does not differ between tumour tissue of G84E variant carriers and non-carriers. Intriguingly, the G84E variant allele was rarely transcribed in carriers, suggesting that HOXB13 expression may be driven by the wild-type allele in the majority of carriers. Nature Publishing Group UK 2017-12-19 /pmc/articles/PMC5736598/ /pubmed/29259341 http://dx.doi.org/10.1038/s41598-017-18217-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article FitzGerald, Liesel M. Raspin, Kelsie Marthick, James R. Field, Matt A. Malley, Roslyn C. Thomson, Russell J. Blackburn, Nicholas B. Banks, Annette Charlesworth, Jac C. Donovan, Shaun Dickinson, Joanne L. Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title | Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title_full | Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title_fullStr | Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title_full_unstemmed | Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title_short | Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
title_sort | impact of the g84e variant on hoxb13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736598/ https://www.ncbi.nlm.nih.gov/pubmed/29259341 http://dx.doi.org/10.1038/s41598-017-18217-w |
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