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Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle
Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-...
Autores principales: | Ikeda, Mitsumi, Matsuyama, Shuichi, Akagi, Satoshi, Ohkoshi, Katsuhiro, Nakamura, Sho, Minabe, Shiori, Kimura, Koji, Hosoe, Misa |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736618/ https://www.ncbi.nlm.nih.gov/pubmed/29259316 http://dx.doi.org/10.1038/s41598-017-17968-w |
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