Cargando…
Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production
Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phom...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736710/ https://www.ncbi.nlm.nih.gov/pubmed/29259312 http://dx.doi.org/10.1038/s41598-017-17895-w |
_version_ | 1783287412763394048 |
---|---|
author | Du, Wen Sun, Chunlong Wang, Jun Xie, Wenjun Wang, Baoqin Liu, Xuehong Zhang, Yumiao Fan, Yanhui |
author_facet | Du, Wen Sun, Chunlong Wang, Jun Xie, Wenjun Wang, Baoqin Liu, Xuehong Zhang, Yumiao Fan, Yanhui |
author_sort | Du, Wen |
collection | PubMed |
description | Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the laccase output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of laccase synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of laccase. The results indicate that the accumulation of superoxide anion radical (O(2) (−)) and hydrogen peroxide (H(2)O(2)) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and chalcone isomerase. This phenomenon is probably the result of the activated phenylpropanoids–flavonoid pathway. Results confirmed that the mixture culture is advantageous for laccase production and revealed that NO, O(2) (−), and H(2)O(2) are necessary signal molecules to induce laccase synthesis. |
format | Online Article Text |
id | pubmed-5736710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57367102017-12-21 Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production Du, Wen Sun, Chunlong Wang, Jun Xie, Wenjun Wang, Baoqin Liu, Xuehong Zhang, Yumiao Fan, Yanhui Sci Rep Article Mixing cultures induces the biosynthesis of laccase in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes laccase production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the laccase output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of laccase synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of laccase. The results indicate that the accumulation of superoxide anion radical (O(2) (−)) and hydrogen peroxide (H(2)O(2)) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and chalcone isomerase. This phenomenon is probably the result of the activated phenylpropanoids–flavonoid pathway. Results confirmed that the mixture culture is advantageous for laccase production and revealed that NO, O(2) (−), and H(2)O(2) are necessary signal molecules to induce laccase synthesis. Nature Publishing Group UK 2017-12-19 /pmc/articles/PMC5736710/ /pubmed/29259312 http://dx.doi.org/10.1038/s41598-017-17895-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Du, Wen Sun, Chunlong Wang, Jun Xie, Wenjun Wang, Baoqin Liu, Xuehong Zhang, Yumiao Fan, Yanhui Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title | Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title_full | Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title_fullStr | Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title_full_unstemmed | Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title_short | Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production |
title_sort | conditions and regulation of mixed culture to promote shiraia bambusicola and phoma sp. bzj6 for laccase production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736710/ https://www.ncbi.nlm.nih.gov/pubmed/29259312 http://dx.doi.org/10.1038/s41598-017-17895-w |
work_keys_str_mv | AT duwen conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT sunchunlong conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT wangjun conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT xiewenjun conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT wangbaoqin conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT liuxuehong conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT zhangyumiao conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction AT fanyanhui conditionsandregulationofmixedculturetopromoteshiraiabambusicolaandphomaspbzj6forlaccaseproduction |