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Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae

Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomi...

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Autores principales: Zhang, Ke, Wu, Xue-Chang, Zheng, Dao-Qiong, Petes, Thomas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736917/
https://www.ncbi.nlm.nih.gov/pubmed/29259092
http://dx.doi.org/10.1128/mBio.02099-17
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author Zhang, Ke
Wu, Xue-Chang
Zheng, Dao-Qiong
Petes, Thomas D.
author_facet Zhang, Ke
Wu, Xue-Chang
Zheng, Dao-Qiong
Petes, Thomas D.
author_sort Zhang, Ke
collection PubMed
description Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures.
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spelling pubmed-57369172017-12-21 Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae Zhang, Ke Wu, Xue-Chang Zheng, Dao-Qiong Petes, Thomas D. mBio Research Article Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures. American Society for Microbiology 2017-12-19 /pmc/articles/PMC5736917/ /pubmed/29259092 http://dx.doi.org/10.1128/mBio.02099-17 Text en Copyright © 2017 Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhang, Ke
Wu, Xue-Chang
Zheng, Dao-Qiong
Petes, Thomas D.
Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title_full Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title_fullStr Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title_full_unstemmed Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title_short Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
title_sort effects of temperature on the meiotic recombination landscape of the yeast saccharomyces cerevisiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736917/
https://www.ncbi.nlm.nih.gov/pubmed/29259092
http://dx.doi.org/10.1128/mBio.02099-17
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