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Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae
Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Microbiology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736917/ https://www.ncbi.nlm.nih.gov/pubmed/29259092 http://dx.doi.org/10.1128/mBio.02099-17 |
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author | Zhang, Ke Wu, Xue-Chang Zheng, Dao-Qiong Petes, Thomas D. |
author_facet | Zhang, Ke Wu, Xue-Chang Zheng, Dao-Qiong Petes, Thomas D. |
author_sort | Zhang, Ke |
collection | PubMed |
description | Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures. |
format | Online Article Text |
id | pubmed-5736917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-57369172017-12-21 Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae Zhang, Ke Wu, Xue-Chang Zheng, Dao-Qiong Petes, Thomas D. mBio Research Article Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures. American Society for Microbiology 2017-12-19 /pmc/articles/PMC5736917/ /pubmed/29259092 http://dx.doi.org/10.1128/mBio.02099-17 Text en Copyright © 2017 Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Zhang, Ke Wu, Xue-Chang Zheng, Dao-Qiong Petes, Thomas D. Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title | Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title_full | Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title_fullStr | Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title_full_unstemmed | Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title_short | Effects of Temperature on the Meiotic Recombination Landscape of the Yeast Saccharomyces cerevisiae |
title_sort | effects of temperature on the meiotic recombination landscape of the yeast saccharomyces cerevisiae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736917/ https://www.ncbi.nlm.nih.gov/pubmed/29259092 http://dx.doi.org/10.1128/mBio.02099-17 |
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