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Role of free DNA ends and protospacer adjacent motifs for CRISPR DNA uptake in Pyrococcus furiosus

To acquire CRISPR–Cas immunity against invasive mobile genetic elements, prokaryotes must first integrate fragments of foreign DNA into their genomic CRISPR arrays for use in future invader silencing. Here, we found that the hyperthermophilic archaeaon, Pyrococcus furiosus, actively incorporates DNA...

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Detalles Bibliográficos
Autores principales:	Shiimori, Masami, Garrett, Sandra C., Chambers, Dwain P., Glover, Claiborne V. C., Graveley, Brenton R., Terns, Michael P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737086/ https://www.ncbi.nlm.nih.gov/pubmed/29036456 http://dx.doi.org/10.1093/nar/gkx839

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