Cargando…

Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)

Glycine, the simplest amino acid in nature and one of the most abundant free amino acids in soil, is regarded as a model nutrient in organic nitrogen studies. To date, many studies have focused on the uptake, metabolism and distribution of organic nitrogen in plants, but few have investigated the nu...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Xiao, Cui, Xiaoxian, Zhao, Li, Guo, Doudou, Feng, Lei, Wei, Shiwei, Zhao, Chao, Huang, Danfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737139/
https://www.ncbi.nlm.nih.gov/pubmed/29326732
http://dx.doi.org/10.3389/fpls.2017.02098
_version_ 1783287480633524224
author Yang, Xiao
Cui, Xiaoxian
Zhao, Li
Guo, Doudou
Feng, Lei
Wei, Shiwei
Zhao, Chao
Huang, Danfeng
author_facet Yang, Xiao
Cui, Xiaoxian
Zhao, Li
Guo, Doudou
Feng, Lei
Wei, Shiwei
Zhao, Chao
Huang, Danfeng
author_sort Yang, Xiao
collection PubMed
description Glycine, the simplest amino acid in nature and one of the most abundant free amino acids in soil, is regarded as a model nutrient in organic nitrogen studies. To date, many studies have focused on the uptake, metabolism and distribution of organic nitrogen in plants, but few have investigated the nutritional performance of plants supplied with organic nitrogen. Lettuce (Lactuca sativa L.), one of the most widely consumed leafy vegetables worldwide, is a significant source of antioxidants and bioactive compounds such as polyphenols, ascorbic acid and tocopherols. In this study, two lettuce cultivars, Shenxuan 1 and Lollo Rossa, were hydroponically cultured in media containing 4.5, 9, or 18 mM glycine or 9 mM nitrate (control) for 4 weeks, and the levels of health-promoting compounds and antioxidant activity of the lettuce leaf extracts were evaluated. Glycine significantly reduced fresh weight compared to control lettuce, while 9 mM glycine significantly increased fresh weight compared to 4.5 or 18 mM glycine. Compared to controls, glycine (18 mM for Shenxuan 1; 9 mM for Lollo Rossa) significantly increased the levels of most antioxidants (including total polyphenols, α-tocopherol) and antioxidant activity, suggesting appropriate glycine supply promotes antioxidant accumulation and activity. Glycine induced most glycosylated quercetin derivatives and luteolin derivatives detected and decreased some phenolic acids compared to nitrate treatment. This study indicates exogenous glycine supplementation could be used strategically to promote the accumulation of health-promoting compounds and antioxidant activity of hydroponically grown lettuce, which could potentially improve human nutrition.
format Online
Article
Text
id pubmed-5737139
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-57371392018-01-11 Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.) Yang, Xiao Cui, Xiaoxian Zhao, Li Guo, Doudou Feng, Lei Wei, Shiwei Zhao, Chao Huang, Danfeng Front Plant Sci Plant Science Glycine, the simplest amino acid in nature and one of the most abundant free amino acids in soil, is regarded as a model nutrient in organic nitrogen studies. To date, many studies have focused on the uptake, metabolism and distribution of organic nitrogen in plants, but few have investigated the nutritional performance of plants supplied with organic nitrogen. Lettuce (Lactuca sativa L.), one of the most widely consumed leafy vegetables worldwide, is a significant source of antioxidants and bioactive compounds such as polyphenols, ascorbic acid and tocopherols. In this study, two lettuce cultivars, Shenxuan 1 and Lollo Rossa, were hydroponically cultured in media containing 4.5, 9, or 18 mM glycine or 9 mM nitrate (control) for 4 weeks, and the levels of health-promoting compounds and antioxidant activity of the lettuce leaf extracts were evaluated. Glycine significantly reduced fresh weight compared to control lettuce, while 9 mM glycine significantly increased fresh weight compared to 4.5 or 18 mM glycine. Compared to controls, glycine (18 mM for Shenxuan 1; 9 mM for Lollo Rossa) significantly increased the levels of most antioxidants (including total polyphenols, α-tocopherol) and antioxidant activity, suggesting appropriate glycine supply promotes antioxidant accumulation and activity. Glycine induced most glycosylated quercetin derivatives and luteolin derivatives detected and decreased some phenolic acids compared to nitrate treatment. This study indicates exogenous glycine supplementation could be used strategically to promote the accumulation of health-promoting compounds and antioxidant activity of hydroponically grown lettuce, which could potentially improve human nutrition. Frontiers Media S.A. 2017-12-15 /pmc/articles/PMC5737139/ /pubmed/29326732 http://dx.doi.org/10.3389/fpls.2017.02098 Text en Copyright © 2017 Yang, Cui, Zhao, Guo, Feng, Wei, Zhao and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Xiao
Cui, Xiaoxian
Zhao, Li
Guo, Doudou
Feng, Lei
Wei, Shiwei
Zhao, Chao
Huang, Danfeng
Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title_full Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title_fullStr Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title_full_unstemmed Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title_short Exogenous Glycine Nitrogen Enhances Accumulation of Glycosylated Flavonoids and Antioxidant Activity in Lettuce (Lactuca sativa L.)
title_sort exogenous glycine nitrogen enhances accumulation of glycosylated flavonoids and antioxidant activity in lettuce (lactuca sativa l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737139/
https://www.ncbi.nlm.nih.gov/pubmed/29326732
http://dx.doi.org/10.3389/fpls.2017.02098
work_keys_str_mv AT yangxiao exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT cuixiaoxian exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT zhaoli exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT guodoudou exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT fenglei exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT weishiwei exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT zhaochao exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival
AT huangdanfeng exogenousglycinenitrogenenhancesaccumulationofglycosylatedflavonoidsandantioxidantactivityinlettucelactucasatival