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Force-activated DNA substrates for probing individual proteins interacting with single-stranded DNA

Single-molecule force spectroscopy provides insight into how proteins bind to and move along DNA. Such studies often embed a single-stranded (ss) DNA region within a longer double-stranded (ds) DNA molecule. Yet, producing these substrates remains laborious and inefficient, particularly when using t...

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Detalles Bibliográficos
Autores principales:	Okoniewski, Stephen R., Uyetake, Lyle, Perkins, Thomas T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737210/ https://www.ncbi.nlm.nih.gov/pubmed/28977580 http://dx.doi.org/10.1093/nar/gkx761

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