Cargando…

Force-activated DNA substrates for probing individual proteins interacting with single-stranded DNA

Single-molecule force spectroscopy provides insight into how proteins bind to and move along DNA. Such studies often embed a single-stranded (ss) DNA region within a longer double-stranded (ds) DNA molecule. Yet, producing these substrates remains laborious and inefficient, particularly when using t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Okoniewski, Stephen R., Uyetake, Lyle, Perkins, Thomas T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737210/ https://www.ncbi.nlm.nih.gov/pubmed/28977580 http://dx.doi.org/10.1093/nar/gkx761

Ejemplares similares

Probing DNA clamps with single-molecule force spectroscopy
por: Wang, Lin, et al.
Publicado: (2013)

HU binds and folds single-stranded DNA
por: Kamashev, Dmitri, et al.
Publicado: (2008)

Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands
por: Sanders, Kelly L., et al.
Publicado: (2009)

Switching from single-stranded to double-stranded DNA limits the unwinding processivity of ring-shaped T7 DNA helicase
por: Jeong, Yong-Joo, et al.
Publicado: (2013)

RNA binding to APOBEC3G induces the disassembly of functional deaminase complexes by displacing single-stranded DNA substrates
por: Polevoda, Bogdan, et al.
Publicado: (2015)

Single molecule analysis of Thermus thermophilus SSB protein dynamics on single-stranded DNA
por: Zhang, Jichuan, et al.
Publicado: (2014)

The interplay of primer-template DNA phosphorylation status and single-stranded DNA binding proteins in directing clamp loaders to the appropriate polarity of DNA
por: Hayner, Jaclyn N., et al.
Publicado: (2014)

The N-terminal domain of TWINKLE contributes to single-stranded DNA binding and DNA helicase activities
por: Farge, Géraldine, et al.
Publicado: (2008)

Physical and functional interaction between yeast Pif1 helicase and Rim1 single-stranded DNA binding protein
por: Ramanagoudr-Bhojappa, Ramanagouda, et al.
Publicado: (2013)

DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase
por: Weinert, Brian T., et al.
Publicado: (2007)

DNA polymerase δ-dependent repair of DNA single strand breaks containing 3′-end proximal lesions
por: Parsons, Jason L., et al.
Publicado: (2007)

Mechanism of strand displacement synthesis by DNA replicative polymerases
por: Manosas, Maria, et al.
Publicado: (2012)

Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNA
por: Galletto, Roberto, et al.
Publicado: (2013)

Single-stranded DNA ligation and XLF-stimulated incompatible DNA end ligation by the XRCC4-DNA ligase IV complex: influence of terminal DNA sequence
por: Gu, Jiafeng, et al.
Publicado: (2007)

Determining the effects of DNA sequence on Hel308 helicase translocation along single-stranded DNA using nanopore tweezers
por: Craig, Jonathan M, et al.
Publicado: (2019)

Topoisomerase II minimizes DNA entanglements by proofreading DNA topology after DNA strand passage
por: Martínez-García, Belén, et al.
Publicado: (2014)

Bulk and single-molecule analysis of a bacterial DNA2-like helicase–nuclease reveals a single-stranded DNA looping motor
por: Wilkinson, Oliver J, et al.
Publicado: (2020)

Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA
por: Torchia, Christopher, et al.
Publicado: (2008)

DNA-binding properties of T4 UvsY recombination mediator protein: polynucleotide wrapping promotes high-affinity binding to single-stranded DNA
por: Xu, Hang, et al.
Publicado: (2010)

Processive translocation mechanism of the human Bloom’s syndrome helicase along single-stranded DNA
por: Gyimesi, Máté, et al.
Publicado: (2010)

A DNA-binding activity in BPV initiator protein E1 required for melting duplex ori DNA but not processive helicase activity initiated on partially single-stranded DNA
por: Sanders, Cyril M.
Publicado: (2008)

Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA
por: Salas, Tonatiuh Romero, et al.
Publicado: (2009)

Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins
por: Guilliam, Thomas A., et al.
Publicado: (2015)

Oligomeric complexes formed by Redβ single strand annealing protein in its different DNA bound states
por: Caldwell, Brian J, et al.
Publicado: (2021)

A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site
por: Sasnauskas, Giedrius, et al.
Publicado: (2010)

A topologically distinct class of photolyases specific for UV lesions within single-stranded DNA
por: Emmerich, Hans-Joachim, et al.
Publicado: (2020)

Microhomology-mediated DNA strand annealing and elongation by human DNA polymerases λ and β on normal and repetitive DNA sequences
por: Crespan, Emmanuele, et al.
Publicado: (2012)

The Hin recombinase assembles a tetrameric protein swivel that exchanges DNA strands
por: Dhar, Gautam, et al.
Publicado: (2009)

Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensis
por: Zhang, Shuli, et al.
Publicado: (2010)

Single-molecule studies of the stringency factors and rates governing the polymerization of RecA on double-stranded DNA
por: Feinstein, Efraim, et al.
Publicado: (2011)

Modifying a covarying protein–DNA interaction changes substrate preference of a site-specific endonuclease
por: Laforet, Marc, et al.
Publicado: (2019)

Molecular basis for DNA strand displacement by NHEJ repair polymerases
por: Bartlett, Edward J., et al.
Publicado: (2016)

Mechanism of DNA substrate recognition by the mammalian DNA repair enzyme, Polynucleotide Kinase
por: Bernstein, N. K., et al.
Publicado: (2009)

Cell cycle regulated DNA methyltransferase: fluorescent tracking of a DNA strand-separation mechanism and identification of the responsible protein motif
por: Konttinen, Olivia, et al.
Publicado: (2020)

Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase
por: Aarattuthodiyil, Suja, et al.
Publicado: (2014)

PCNA and XPF cooperate to distort DNA substrates
por: Hutton, Richard D., et al.
Publicado: (2010)

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase
por: Hamilton, Michael D., et al.
Publicado: (2007)

High fidelity DNA strand-separation is the major specificity determinant in DNA methyltransferase CcrM’s catalytic mechanism
por: Konttinen, Olivia, et al.
Publicado: (2023)

The yeast Pif1p DNA helicase preferentially unwinds RNA–DNA substrates
por: Boulé, Jean-Baptiste, et al.
Publicado: (2007)

Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase
por: Craggs, Timothy D, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_jda1qcqbtvg9kna5iem2dskg9a