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Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging
Organic dyes generally suffer from small Stokes shift that usually leads to self‐quenching and ‐gaining errors during the fluorescent imaging process. Here, a through‐bond energy transfer (TBET) cassette is developed with large Stokes shift to pursue precise cell imaging. The TBET is constructed by...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737226/ https://www.ncbi.nlm.nih.gov/pubmed/29270336 http://dx.doi.org/10.1002/advs.201700229 |
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author | Xue, Xiangdong Jin, Shubin Li, Zhipeng Zhang, Chunqiu Guo, Weisheng Hu, Liming Wang, Paul C. Zhang, Jinchao Liang, Xing‐Jie |
author_facet | Xue, Xiangdong Jin, Shubin Li, Zhipeng Zhang, Chunqiu Guo, Weisheng Hu, Liming Wang, Paul C. Zhang, Jinchao Liang, Xing‐Jie |
author_sort | Xue, Xiangdong |
collection | PubMed |
description | Organic dyes generally suffer from small Stokes shift that usually leads to self‐quenching and ‐gaining errors during the fluorescent imaging process. Here, a through‐bond energy transfer (TBET) cassette is developed with large Stokes shift to pursue precise cell imaging. The TBET is constructed by covalently conjugated tetraphenylethene (acts as donor) and rhodamine (acceptor) through an acetylene bond. The constructed TBET cassette distinctly behaves as dual‐Stokes shifts, including a large pseudo‐Stokes shift caused by energy transfer, from donor's absorption to acceptor's emission (up to 260 nm) and a smaller Stokes shift of acceptor molecules itself. Due to the intrinsic dual‐Stokes shifts, TBET cassette exhibits specific “dual distinct absorbances, single shared emission” properties, which can be excitated under two different laser channels. By colocalization of the imaging readouts of these two channels, the precisely “double checked” fluorescent imaging is achieved in living cells. |
format | Online Article Text |
id | pubmed-5737226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57372262017-12-21 Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging Xue, Xiangdong Jin, Shubin Li, Zhipeng Zhang, Chunqiu Guo, Weisheng Hu, Liming Wang, Paul C. Zhang, Jinchao Liang, Xing‐Jie Adv Sci (Weinh) Communications Organic dyes generally suffer from small Stokes shift that usually leads to self‐quenching and ‐gaining errors during the fluorescent imaging process. Here, a through‐bond energy transfer (TBET) cassette is developed with large Stokes shift to pursue precise cell imaging. The TBET is constructed by covalently conjugated tetraphenylethene (acts as donor) and rhodamine (acceptor) through an acetylene bond. The constructed TBET cassette distinctly behaves as dual‐Stokes shifts, including a large pseudo‐Stokes shift caused by energy transfer, from donor's absorption to acceptor's emission (up to 260 nm) and a smaller Stokes shift of acceptor molecules itself. Due to the intrinsic dual‐Stokes shifts, TBET cassette exhibits specific “dual distinct absorbances, single shared emission” properties, which can be excitated under two different laser channels. By colocalization of the imaging readouts of these two channels, the precisely “double checked” fluorescent imaging is achieved in living cells. John Wiley and Sons Inc. 2017-10-27 /pmc/articles/PMC5737226/ /pubmed/29270336 http://dx.doi.org/10.1002/advs.201700229 Text en © 2017 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications Xue, Xiangdong Jin, Shubin Li, Zhipeng Zhang, Chunqiu Guo, Weisheng Hu, Liming Wang, Paul C. Zhang, Jinchao Liang, Xing‐Jie Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title | Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title_full | Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title_fullStr | Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title_full_unstemmed | Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title_short | Through‐Bond Energy Transfer Cassette with Dual‐Stokes Shifts for “Double Checked” Cell Imaging |
title_sort | through‐bond energy transfer cassette with dual‐stokes shifts for “double checked” cell imaging |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737226/ https://www.ncbi.nlm.nih.gov/pubmed/29270336 http://dx.doi.org/10.1002/advs.201700229 |
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