A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells

Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5′-TTTN-3′ protospacer adjacent motif (PAM) at the 5′ end of the protospacer for genome editing. For widesp...

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Detalles Bibliográficos
Autores principales: Tu, Mengjun, Lin, Li, Cheng, Yilu, He, Xiubin, Sun, Huihui, Xie, Haihua, Fu, Junhao, Liu, Changbao, Li, Jin, Chen, Ding, Xi, Haitao, Xue, Dongyu, Liu, Qi, Zhao, Junzhao, Gao, Caixia, Song, Zongming, Qu, Jia, Gu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737432/
https://www.ncbi.nlm.nih.gov/pubmed/28977650
http://dx.doi.org/10.1093/nar/gkx783

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