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De novo design and synthesis of a 30-cistron translation-factor module
Two of the many goals of synthetic biology are synthesizing large biochemical systems and simplifying their assembly. While several genes have been assembled together by modular idempotent cloning, it is unclear if such simplified strategies scale to very large constructs for expression and purifica...
Autores principales: | Shepherd, Tyson R., Du, Liping, Liljeruhm, Josefine, Samudyata, Wang, Jinfan, Sjödin, Marcus O.D., Wetterhall, Magnus, Yomo, Tetsuya, Forster, Anthony C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737471/ https://www.ncbi.nlm.nih.gov/pubmed/28977654 http://dx.doi.org/10.1093/nar/gkx753 |
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