Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity

The retargeting of protein–DNA specificity, outside of extremely modular DNA binding proteins such as TAL effectors, has generally proved to be quite challenging. Here, we describe structural analyses of five different extensively retargeted variants of a single homing endonuclease, that have been s...

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Detalles Bibliográficos
Autores principales: Werther, Rachel, Hallinan, Jazmine P., Lambert, Abigail R., Havens, Kyle, Pogson, Mark, Jarjour, Jordan, Galizi, Roberto, Windbichler, Nikolai, Crisanti, Andrea, Nolan, Tony, Stoddard, Barry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737575/
https://www.ncbi.nlm.nih.gov/pubmed/28637173
http://dx.doi.org/10.1093/nar/gkx544

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