Cargando…

Oxidative stress rapidly stabilizes promoter-proximal paused Pol II across the human genome

Oxidative stress has pervasive effects on cells but how they respond transcriptionally upon the initial insult is incompletely understood. We developed a nuclear walk-on assay that semi-globally quantifies nascent transcripts in promoter-proximal paused RNA polymerase II (Pol II). Using this assay i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nilson, Kyle A., Lawson, Christine K., Mullen, Nicholas J., Ball, Christopher B., Spector, Benjamin M., Meier, Jeffery L., Price, David H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Gene regulation, Chromatin and Epigenetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5737879/ https://www.ncbi.nlm.nih.gov/pubmed/28977633 http://dx.doi.org/10.1093/nar/gkx724

Ejemplares similares

Release of promoter–proximal paused Pol II in response to histone deacetylase inhibition
por: Vaid, Roshan, et al.
Publicado: (2020)

SAYP and Brahma are important for ‘repressive’ and ‘transient’ Pol II pausing
por: Vorobyeva, Nadezhda E., et al.
Publicado: (2012)

A positioned +1 nucleosome enhances promoter-proximal pausing
por: Jimeno-González, Silvia, et al.
Publicado: (2015)

σ(38)-dependent promoter-proximal pausing by bacterial RNA polymerase
por: Petushkov, Ivan, et al.
Publicado: (2017)

G4 motifs correlate with promoter-proximal transcriptional pausing in human genes
por: Eddy, Johanna, et al.
Publicado: (2011)

A universal transcription pause sequence is an element of initiation factor σ70-dependent pausing
por: Bird, Jeremy G., et al.
Publicado: (2016)

A unified view of the sequence and functional organization of the human RNA polymerase II promoter
por: Luse, Donal S, et al.
Publicado: (2020)

Control of VEGF-A transcriptional programs by pausing and genomic compartmentalization
por: Kaikkonen, Minna U., et al.
Publicado: (2014)

Misincorporation by RNA polymerase is a major source of transcription pausing in vivo
por: James, Katherine, et al.
Publicado: (2017)

Conserved DNA sequence features underlie pervasive RNA polymerase pausing
por: Gajos, Martyna, et al.
Publicado: (2021)

TFII-I-mediated polymerase pausing antagonizes GLI2 induction by TGFβ
por: McCleary-Wheeler, Angela L, et al.
Publicado: (2020)

Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing
por: Pérez-Lluch, Sílvia, et al.
Publicado: (2011)

Splicing inhibition decreases phosphorylation level of Ser2 in Pol II CTD
por: Koga, Mitsunori, et al.
Publicado: (2015)

Wide-ranging and unexpected consequences of altered Pol II catalytic activity in vivo
por: Malik, Indranil, et al.
Publicado: (2017)

Estrogen receptor-α recruits P-TEFb to overcome transcriptional pausing in intron 1 of the MYB gene
por: Mitra, Partha, et al.
Publicado: (2012)

RNA polymerase II pausing can be retained or acquired during activation of genes involved in the epithelial to mesenchymal transition
por: Samarakkody, Ann, et al.
Publicado: (2015)

StpA and Hha stimulate pausing by RNA polymerase by promoting DNA–DNA bridging of H-NS filaments
por: Boudreau, Beth A, et al.
Publicado: (2018)

Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release
por: Wang, Zhijia, et al.
Publicado: (2023)

The NRPD1 N-terminus contains a Pol IV-specific motif that is critical for genome surveillance in Arabidopsis
por: Ferrafiat, Laura, et al.
Publicado: (2019)

Nuclear export restricts Gdown1 to a mitotic function
por: Ball, Christopher B, et al.
Publicado: (2022)

Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe
por: Sansó, Miriam, et al.
Publicado: (2011)

Schizosaccharomyces pombe Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex
por: Gopalan, Sneha, et al.
Publicado: (2018)

The Pol IV largest subunit CTD quantitatively affects siRNA levels guiding RNA-directed DNA methylation
por: Wendte, Jered M, et al.
Publicado: (2019)

Expression of Bacillus subtilis ABCF antibiotic resistance factor VmlR is regulated by RNA polymerase pausing, transcription attenuation, translation attenuation and (p)ppGpp
por: Takada, Hiraku, et al.
Publicado: (2022)

A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation
por: Dronamraju, Raghuvar, et al.
Publicado: (2014)

Requirement of TFIIH kinase subunit Mat1 for RNA Pol II C-terminal domain Ser5 phosphorylation, transcription and mRNA turnover
por: Helenius, Katja, et al.
Publicado: (2011)

Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS
por: Tao, Yongguang, et al.
Publicado: (2011)

The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs
por: Dou, Kun, et al.
Publicado: (2013)

Oct-2 forms a complex with Oct-1 on the iNOS promoter and represses transcription by interfering with recruitment of RNA PolII by Oct-1
por: Bentrari, Fatima, et al.
Publicado: (2015)

Genetic screen for suppression of transcriptional interference reveals fission yeast 14–3–3 protein Rad24 as an antagonist of precocious Pol2 transcription termination
por: Garg, Angad, et al.
Publicado: (2021)

TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production
por: Philippi, Anja, et al.
Publicado: (2010)

Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding
por: Maruyama, Atsushi, et al.
Publicado: (2014)

A genetic screen for suppressors of hyper-repression of the fission yeast PHO regulon by Pol2 CTD mutation T4A implicates inositol 1-pyrophosphates as agonists of precocious lncRNA transcription termination
por: Garg, Angad, et al.
Publicado: (2020)

Analysis of epigenetic stability and conversions in Saccharomyces cerevisiae reveals a novel role of CAF-I in position-effect variegation
por: Jeffery, Daniel C. B., et al.
Publicado: (2013)

A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation
por: Kochanova, Natalia Y, et al.
Publicado: (2020)

INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes
por: Klopf, Eva, et al.
Publicado: (2017)

Disclosure of a structural milieu for the proximity ligation reveals the elusive nature of an active chromatin hub
por: Gavrilov, Alexey A., et al.
Publicado: (2013)

The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region
por: Yang, Silu, et al.
Publicado: (2014)

Promoter-proximal transcription factor binding is transcriptionally active when coupled with nucleosome repositioning in immediate vicinity
por: Yadav, Vinod Kumar, et al.
Publicado: (2014)

Effects of DNA methylation on nucleosome stability
por: Collings, Clayton K., et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_k4a25s7tn2t4eu3tjqebe9e9e1