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In vitro dynamic model simulating the digestive tract of 6-month-old infants
BACKGROUND: In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in infants, could be of great help in preventing food-related pathologies and in developing new formulas with health benef...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738106/ https://www.ncbi.nlm.nih.gov/pubmed/29261742 http://dx.doi.org/10.1371/journal.pone.0189807 |
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author | Passannanti, Francesca Nigro, Federica Gallo, Marianna Tornatore, Fabio Frasso, Annalisa Saccone, Giulia Budelli, Andrea Barone, Maria V. Nigro, Roberto |
author_facet | Passannanti, Francesca Nigro, Federica Gallo, Marianna Tornatore, Fabio Frasso, Annalisa Saccone, Giulia Budelli, Andrea Barone, Maria V. Nigro, Roberto |
author_sort | Passannanti, Francesca |
collection | PubMed |
description | BACKGROUND: In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in infants, could be of great help in preventing food-related pathologies and in developing new formulas with health benefits. In this context, in vitro dynamic systems to simulate human digestion and, in particular, infant digestion could become increasingly valuable. OBJECTIVE: To simulate the digestive process through the use of a dynamic model of the infant gastroenteric apparatus to study the digestibility of starch-based infant foods. DESIGN: Using M.I.D.A (Model of an Infant Digestive Apparatus), the oral, gastric and intestinal digestibility of two starch-based products were measured: 1) rice starch mixed with distilled water and treated using two different sterilization methods (the classical method with a holding temperature of 121°C for 37 min and the HTST method with a holding temperature of 137°C for 70 sec) and 2) a rice cream with (premium product) or without (basic product) an aliquot of rice flour fermented by Lactobacillus paracasei CBA L74. After the digestion the foods were analyzed for the starch concentration, the amount of D-glucose released and the percentage of hydrolyzed starch. RESULTS: An in vitro dynamic system, which was referred to as M.I.D.A., was obtained. Using this system, the starch digestion occurred only during the oral and intestinal phase, as expected. The D-glucose released during the intestinal phase was different between the classical and HTST methods (0.795 grams for the HTST versus 0.512 for the classical product). The same analysis was performed for the basic and premium products. In this case, the premium product had a significant difference in terms of the starch hydrolysis percentage during the entire process. CONCLUSIONS: The M.I.D.A. system was able to digest simple starches and a more complex food in the correct compartments. In this study, better digestibility of the premium product was revealed. |
format | Online Article Text |
id | pubmed-5738106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57381062017-12-29 In vitro dynamic model simulating the digestive tract of 6-month-old infants Passannanti, Francesca Nigro, Federica Gallo, Marianna Tornatore, Fabio Frasso, Annalisa Saccone, Giulia Budelli, Andrea Barone, Maria V. Nigro, Roberto PLoS One Research Article BACKGROUND: In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in infants, could be of great help in preventing food-related pathologies and in developing new formulas with health benefits. In this context, in vitro dynamic systems to simulate human digestion and, in particular, infant digestion could become increasingly valuable. OBJECTIVE: To simulate the digestive process through the use of a dynamic model of the infant gastroenteric apparatus to study the digestibility of starch-based infant foods. DESIGN: Using M.I.D.A (Model of an Infant Digestive Apparatus), the oral, gastric and intestinal digestibility of two starch-based products were measured: 1) rice starch mixed with distilled water and treated using two different sterilization methods (the classical method with a holding temperature of 121°C for 37 min and the HTST method with a holding temperature of 137°C for 70 sec) and 2) a rice cream with (premium product) or without (basic product) an aliquot of rice flour fermented by Lactobacillus paracasei CBA L74. After the digestion the foods were analyzed for the starch concentration, the amount of D-glucose released and the percentage of hydrolyzed starch. RESULTS: An in vitro dynamic system, which was referred to as M.I.D.A., was obtained. Using this system, the starch digestion occurred only during the oral and intestinal phase, as expected. The D-glucose released during the intestinal phase was different between the classical and HTST methods (0.795 grams for the HTST versus 0.512 for the classical product). The same analysis was performed for the basic and premium products. In this case, the premium product had a significant difference in terms of the starch hydrolysis percentage during the entire process. CONCLUSIONS: The M.I.D.A. system was able to digest simple starches and a more complex food in the correct compartments. In this study, better digestibility of the premium product was revealed. Public Library of Science 2017-12-20 /pmc/articles/PMC5738106/ /pubmed/29261742 http://dx.doi.org/10.1371/journal.pone.0189807 Text en © 2017 Passannanti et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Passannanti, Francesca Nigro, Federica Gallo, Marianna Tornatore, Fabio Frasso, Annalisa Saccone, Giulia Budelli, Andrea Barone, Maria V. Nigro, Roberto In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title | In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title_full | In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title_fullStr | In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title_full_unstemmed | In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title_short | In vitro dynamic model simulating the digestive tract of 6-month-old infants |
title_sort | in vitro dynamic model simulating the digestive tract of 6-month-old infants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738106/ https://www.ncbi.nlm.nih.gov/pubmed/29261742 http://dx.doi.org/10.1371/journal.pone.0189807 |
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