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T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with N...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738582/ https://www.ncbi.nlm.nih.gov/pubmed/29362561 http://dx.doi.org/10.1155/2017/5023680 |
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author | Losurdo, Giuseppe Piscitelli, Domenico Pezzuto, Federica Fortarezza, Francesco Covelli, Claudia Marra, Antonella Iannone, Andrea Amoruso, Annacinzia Principi, Mariabeatrice Ierardi, Enzo Di Leo, Alfredo |
author_facet | Losurdo, Giuseppe Piscitelli, Domenico Pezzuto, Federica Fortarezza, Francesco Covelli, Claudia Marra, Antonella Iannone, Andrea Amoruso, Annacinzia Principi, Mariabeatrice Ierardi, Enzo Di Leo, Alfredo |
author_sort | Losurdo, Giuseppe |
collection | PubMed |
description | BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni's tests were used for statistical analysis. RESULTS: Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p < 0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm(2); p < 0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p < 0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm(2); p = 0.009). CONCLUSIONS: The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS. |
format | Online Article Text |
id | pubmed-5738582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-57385822018-01-23 T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity Losurdo, Giuseppe Piscitelli, Domenico Pezzuto, Federica Fortarezza, Francesco Covelli, Claudia Marra, Antonella Iannone, Andrea Amoruso, Annacinzia Principi, Mariabeatrice Ierardi, Enzo Di Leo, Alfredo Gastroenterol Res Pract Research Article BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni's tests were used for statistical analysis. RESULTS: Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p < 0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm(2); p < 0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p < 0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm(2); p = 0.009). CONCLUSIONS: The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS. Hindawi 2017 2017-12-07 /pmc/articles/PMC5738582/ /pubmed/29362561 http://dx.doi.org/10.1155/2017/5023680 Text en Copyright © 2017 Giuseppe Losurdo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Losurdo, Giuseppe Piscitelli, Domenico Pezzuto, Federica Fortarezza, Francesco Covelli, Claudia Marra, Antonella Iannone, Andrea Amoruso, Annacinzia Principi, Mariabeatrice Ierardi, Enzo Di Leo, Alfredo T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title_full | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title_fullStr | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title_full_unstemmed | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title_short | T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity |
title_sort | t helper lymphocyte and mast cell immunohistochemical pattern in nonceliac gluten sensitivity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738582/ https://www.ncbi.nlm.nih.gov/pubmed/29362561 http://dx.doi.org/10.1155/2017/5023680 |
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