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T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity

BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with N...

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Autores principales: Losurdo, Giuseppe, Piscitelli, Domenico, Pezzuto, Federica, Fortarezza, Francesco, Covelli, Claudia, Marra, Antonella, Iannone, Andrea, Amoruso, Annacinzia, Principi, Mariabeatrice, Ierardi, Enzo, Di Leo, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738582/
https://www.ncbi.nlm.nih.gov/pubmed/29362561
http://dx.doi.org/10.1155/2017/5023680
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author Losurdo, Giuseppe
Piscitelli, Domenico
Pezzuto, Federica
Fortarezza, Francesco
Covelli, Claudia
Marra, Antonella
Iannone, Andrea
Amoruso, Annacinzia
Principi, Mariabeatrice
Ierardi, Enzo
Di Leo, Alfredo
author_facet Losurdo, Giuseppe
Piscitelli, Domenico
Pezzuto, Federica
Fortarezza, Francesco
Covelli, Claudia
Marra, Antonella
Iannone, Andrea
Amoruso, Annacinzia
Principi, Mariabeatrice
Ierardi, Enzo
Di Leo, Alfredo
author_sort Losurdo, Giuseppe
collection PubMed
description BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni's tests were used for statistical analysis. RESULTS: Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p < 0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm(2); p < 0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p < 0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm(2); p = 0.009). CONCLUSIONS: The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS.
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spelling pubmed-57385822018-01-23 T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity Losurdo, Giuseppe Piscitelli, Domenico Pezzuto, Federica Fortarezza, Francesco Covelli, Claudia Marra, Antonella Iannone, Andrea Amoruso, Annacinzia Principi, Mariabeatrice Ierardi, Enzo Di Leo, Alfredo Gastroenterol Res Pract Research Article BACKGROUND AND AIMS: Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition. Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation. MATERIALS AND METHODS: We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria. We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls. Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed. ANOVA plus Bonferroni's tests were used for statistical analysis. RESULTS: Twenty NCGS, 16 celiac disease, and 16 negative controls were selected. CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p < 0.001). CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm(2); p < 0.001). CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p < 0.001). CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm(2); p = 0.009). CONCLUSIONS: The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS. Hindawi 2017 2017-12-07 /pmc/articles/PMC5738582/ /pubmed/29362561 http://dx.doi.org/10.1155/2017/5023680 Text en Copyright © 2017 Giuseppe Losurdo et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Losurdo, Giuseppe
Piscitelli, Domenico
Pezzuto, Federica
Fortarezza, Francesco
Covelli, Claudia
Marra, Antonella
Iannone, Andrea
Amoruso, Annacinzia
Principi, Mariabeatrice
Ierardi, Enzo
Di Leo, Alfredo
T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title_full T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title_fullStr T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title_full_unstemmed T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title_short T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
title_sort t helper lymphocyte and mast cell immunohistochemical pattern in nonceliac gluten sensitivity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738582/
https://www.ncbi.nlm.nih.gov/pubmed/29362561
http://dx.doi.org/10.1155/2017/5023680
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