Cargando…

Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil

The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candidu...

Descripción completa

Detalles Bibliográficos
Autores principales: Lima, Gláucia Moreira Espíndola, Nunes, Maína de Oliveira, Chang, Marilene Rodrigues, Tsujisaki, Rosianne Assis de Sousa, Nunes, Joslaine de Oliveira, Taira, Cleison Ledesma, Thomaz, Danilo Yamamoto, Negro, Gilda Maria Bárbaro Del, Mendes, Rinaldo Pôncio, Paniago, Anamaria Mello Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738760/
https://www.ncbi.nlm.nih.gov/pubmed/29267583
http://dx.doi.org/10.1590/S1678-9946201759075
_version_ 1783287753023160320
author Lima, Gláucia Moreira Espíndola
Nunes, Maína de Oliveira
Chang, Marilene Rodrigues
Tsujisaki, Rosianne Assis de Sousa
Nunes, Joslaine de Oliveira
Taira, Cleison Ledesma
Thomaz, Danilo Yamamoto
Negro, Gilda Maria Bárbaro Del
Mendes, Rinaldo Pôncio
Paniago, Anamaria Mello Miranda
author_facet Lima, Gláucia Moreira Espíndola
Nunes, Maína de Oliveira
Chang, Marilene Rodrigues
Tsujisaki, Rosianne Assis de Sousa
Nunes, Joslaine de Oliveira
Taira, Cleison Ledesma
Thomaz, Danilo Yamamoto
Negro, Gilda Maria Bárbaro Del
Mendes, Rinaldo Pôncio
Paniago, Anamaria Mello Miranda
author_sort Lima, Gláucia Moreira Espíndola
collection PubMed
description The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) - 33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria.
format Online
Article
Text
id pubmed-5738760
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Instituto de Medicina Tropical
record_format MEDLINE/PubMed
spelling pubmed-57387602018-01-02 Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil Lima, Gláucia Moreira Espíndola Nunes, Maína de Oliveira Chang, Marilene Rodrigues Tsujisaki, Rosianne Assis de Sousa Nunes, Joslaine de Oliveira Taira, Cleison Ledesma Thomaz, Danilo Yamamoto Negro, Gilda Maria Bárbaro Del Mendes, Rinaldo Pôncio Paniago, Anamaria Mello Miranda Rev Inst Med Trop Sao Paulo Original Article The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) - 33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria. Instituto de Medicina Tropical 2017-12-21 /pmc/articles/PMC5738760/ /pubmed/29267583 http://dx.doi.org/10.1590/S1678-9946201759075 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lima, Gláucia Moreira Espíndola
Nunes, Maína de Oliveira
Chang, Marilene Rodrigues
Tsujisaki, Rosianne Assis de Sousa
Nunes, Joslaine de Oliveira
Taira, Cleison Ledesma
Thomaz, Danilo Yamamoto
Negro, Gilda Maria Bárbaro Del
Mendes, Rinaldo Pôncio
Paniago, Anamaria Mello Miranda
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title_full Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title_fullStr Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title_full_unstemmed Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title_short Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
title_sort identification and antifungal susceptibility of candida species isolated from the urine of patients in a university hospital in brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738760/
https://www.ncbi.nlm.nih.gov/pubmed/29267583
http://dx.doi.org/10.1590/S1678-9946201759075
work_keys_str_mv AT limaglauciamoreiraespindola identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT nunesmainadeoliveira identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT changmarilenerodrigues identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT tsujisakirosianneassisdesousa identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT nunesjoslainedeoliveira identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT tairacleisonledesma identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT thomazdaniloyamamoto identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT negrogildamariabarbarodel identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT mendesrinaldoponcio identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil
AT paniagoanamariamellomiranda identificationandantifungalsusceptibilityofcandidaspeciesisolatedfromtheurineofpatientsinauniversityhospitalinbrazil