Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

A Lateral Flow Assay to detect cryptococcal antigen (CrAg(®) LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg(®) LFA was 100%. High concordance (kappa index=1.0) between Cr...

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Detalles Bibliográficos
Autores principales: Cáceres, Diego H., Zuluaga, Alejandra, Tabares, Ángela M., Chiller, Tom, González, Ángel, Gómez, Beatriz L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2017
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738761/
https://www.ncbi.nlm.nih.gov/pubmed/29267584
http://dx.doi.org/10.1590/S1678-9946201759076
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author Cáceres, Diego H.
Zuluaga, Alejandra
Tabares, Ángela M.
Chiller, Tom
González, Ángel
Gómez, Beatriz L.
author_facet Cáceres, Diego H.
Zuluaga, Alejandra
Tabares, Ángela M.
Chiller, Tom
González, Ángel
Gómez, Beatriz L.
author_sort Cáceres, Diego H.
collection PubMed
description A Lateral Flow Assay to detect cryptococcal antigen (CrAg(®) LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg(®) LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS(®)) and CrAg(®) LFA was observed. CrAg(®) LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.
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spelling pubmed-57387612018-01-02 Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia Cáceres, Diego H. Zuluaga, Alejandra Tabares, Ángela M. Chiller, Tom González, Ángel Gómez, Beatriz L. Rev Inst Med Trop Sao Paulo Brief Communication A Lateral Flow Assay to detect cryptococcal antigen (CrAg(®) LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg(®) LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS(®)) and CrAg(®) LFA was observed. CrAg(®) LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen. Instituto de Medicina Tropical 2017-12-21 /pmc/articles/PMC5738761/ /pubmed/29267584 http://dx.doi.org/10.1590/S1678-9946201759076 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Cáceres, Diego H.
Zuluaga, Alejandra
Tabares, Ángela M.
Chiller, Tom
González, Ángel
Gómez, Beatriz L.
Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_full Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_fullStr Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_full_unstemmed Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_short Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia
title_sort evaluation of a cryptococcal antigen lateral flow assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in colombia
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738761/
https://www.ncbi.nlm.nih.gov/pubmed/29267584
http://dx.doi.org/10.1590/S1678-9946201759076
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