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A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus

OBJECTIVES: Seabirds have suffered dramatic population declines in recent decades with one such species being the sooty tern Onychoprion fuscatus. An urgent call to re-assess their conservation status has been made given that some populations, such as the one on Ascension Island, South Atlantic, hav...

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Autores principales: Garrett, Lucy J. H., Dawson, Deborah A., Horsburgh, Gavin J., Reynolds, Silas James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738816/
https://www.ncbi.nlm.nih.gov/pubmed/29262842
http://dx.doi.org/10.1186/s13104-017-3084-9
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author Garrett, Lucy J. H.
Dawson, Deborah A.
Horsburgh, Gavin J.
Reynolds, Silas James
author_facet Garrett, Lucy J. H.
Dawson, Deborah A.
Horsburgh, Gavin J.
Reynolds, Silas James
author_sort Garrett, Lucy J. H.
collection PubMed
description OBJECTIVES: Seabirds have suffered dramatic population declines in recent decades with one such species being the sooty tern Onychoprion fuscatus. An urgent call to re-assess their conservation status has been made given that some populations, such as the one on Ascension Island, South Atlantic, have declined by over 80% in three generations. Little is known about their population genetics, which would aid conservation management through understanding ecological processes and vulnerability to environmental change. We developed a multiplex microsatellite marker set for sooty terns including sex-typing markers to assist population genetics studies. RESULTS: Fifty microsatellite loci were isolated and tested in 23 individuals from Ascension Island. Thirty-one were polymorphic and displayed between 4 and 20 alleles. Three loci were Z-linked and two autosomal loci deviated from Hardy–Weinberg equilibrium. The remaining 26 autosomal loci together with three sex-typing makers were optimised in seven polymerase chain reaction plexes. These 26 highly polymorphic markers will be useful for understanding genetic structure of the Ascension Island population and the species as a whole. Combining these with recently developed microsatellite markers isolated from Indian Ocean birds will allow for assessment of global population structure and genetic diversity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-3084-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-57388162018-01-02 A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus Garrett, Lucy J. H. Dawson, Deborah A. Horsburgh, Gavin J. Reynolds, Silas James BMC Res Notes Research Note OBJECTIVES: Seabirds have suffered dramatic population declines in recent decades with one such species being the sooty tern Onychoprion fuscatus. An urgent call to re-assess their conservation status has been made given that some populations, such as the one on Ascension Island, South Atlantic, have declined by over 80% in three generations. Little is known about their population genetics, which would aid conservation management through understanding ecological processes and vulnerability to environmental change. We developed a multiplex microsatellite marker set for sooty terns including sex-typing markers to assist population genetics studies. RESULTS: Fifty microsatellite loci were isolated and tested in 23 individuals from Ascension Island. Thirty-one were polymorphic and displayed between 4 and 20 alleles. Three loci were Z-linked and two autosomal loci deviated from Hardy–Weinberg equilibrium. The remaining 26 autosomal loci together with three sex-typing makers were optimised in seven polymerase chain reaction plexes. These 26 highly polymorphic markers will be useful for understanding genetic structure of the Ascension Island population and the species as a whole. Combining these with recently developed microsatellite markers isolated from Indian Ocean birds will allow for assessment of global population structure and genetic diversity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-017-3084-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-20 /pmc/articles/PMC5738816/ /pubmed/29262842 http://dx.doi.org/10.1186/s13104-017-3084-9 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Garrett, Lucy J. H.
Dawson, Deborah A.
Horsburgh, Gavin J.
Reynolds, Silas James
A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title_full A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title_fullStr A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title_full_unstemmed A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title_short A multiplex marker set for microsatellite typing and sexing of sooty terns Onychoprion fuscatus
title_sort multiplex marker set for microsatellite typing and sexing of sooty terns onychoprion fuscatus
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738816/
https://www.ncbi.nlm.nih.gov/pubmed/29262842
http://dx.doi.org/10.1186/s13104-017-3084-9
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