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Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences
Mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. Here we show that different Sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738999/ https://www.ncbi.nlm.nih.gov/pubmed/28800133 http://dx.doi.org/10.1038/ismej.2017.136 |
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author | Zhao, Ran Liu, Li Xue Zhang, Yun Zeng Jiao, Jian Cui, Wen Jing Zhang, Biliang Wang, Xiao Lin Li, Meng Lin Chen, Yi Xiong, Zhu Qing Chen, Wen Xin Tian, Chang Fu |
author_facet | Zhao, Ran Liu, Li Xue Zhang, Yun Zeng Jiao, Jian Cui, Wen Jing Zhang, Biliang Wang, Xiao Lin Li, Meng Lin Chen, Yi Xiong, Zhu Qing Chen, Wen Xin Tian, Chang Fu |
author_sort | Zhao, Ran |
collection | PubMed |
description | Mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. Here we show that different Sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, while being all effective microsymbionts of some wild soybeans. However, a few well-infected nodules can be found on a commercial soybean using inocula containing a mixed pool of Tn5 insertion mutants derived from an incompatible strain. Reverse genetics and genome sequencing of compatible mutants demonstrated that inactivation of T3SS (type three secretion system) accounted for this phenotypic change. These mutations in the T3SS gene cluster were dominated by parallel transpositions of insertion sequences (ISs) other than the introduced Tn5. This genetic and phenotypic change can also be achieved in an experimental evolution scenario on a laboratory time scale using incompatible wild-type strains as inocula. The ISs acting in the adaptive evolution of Sinorhizobium strains exhibit broader phyletic and replicon distributions than other ISs, and prefer target sequences of low GC% content, a characteristic feature of symbiosis plasmid where T3SS genes are located. These findings suggest an important role of co-evolved ISs in the adaptive evolution of rhizobial compatibility. |
format | Online Article Text |
id | pubmed-5738999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-57389992018-01-01 Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences Zhao, Ran Liu, Li Xue Zhang, Yun Zeng Jiao, Jian Cui, Wen Jing Zhang, Biliang Wang, Xiao Lin Li, Meng Lin Chen, Yi Xiong, Zhu Qing Chen, Wen Xin Tian, Chang Fu ISME J Original Article Mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. Here we show that different Sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, while being all effective microsymbionts of some wild soybeans. However, a few well-infected nodules can be found on a commercial soybean using inocula containing a mixed pool of Tn5 insertion mutants derived from an incompatible strain. Reverse genetics and genome sequencing of compatible mutants demonstrated that inactivation of T3SS (type three secretion system) accounted for this phenotypic change. These mutations in the T3SS gene cluster were dominated by parallel transpositions of insertion sequences (ISs) other than the introduced Tn5. This genetic and phenotypic change can also be achieved in an experimental evolution scenario on a laboratory time scale using incompatible wild-type strains as inocula. The ISs acting in the adaptive evolution of Sinorhizobium strains exhibit broader phyletic and replicon distributions than other ISs, and prefer target sequences of low GC% content, a characteristic feature of symbiosis plasmid where T3SS genes are located. These findings suggest an important role of co-evolved ISs in the adaptive evolution of rhizobial compatibility. Nature Publishing Group 2018-01 2017-08-11 /pmc/articles/PMC5738999/ /pubmed/28800133 http://dx.doi.org/10.1038/ismej.2017.136 Text en Copyright © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Original Article Zhao, Ran Liu, Li Xue Zhang, Yun Zeng Jiao, Jian Cui, Wen Jing Zhang, Biliang Wang, Xiao Lin Li, Meng Lin Chen, Yi Xiong, Zhu Qing Chen, Wen Xin Tian, Chang Fu Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title | Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title_full | Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title_fullStr | Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title_full_unstemmed | Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title_short | Adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
title_sort | adaptive evolution of rhizobial symbiotic compatibility mediated by co-evolved insertion sequences |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5738999/ https://www.ncbi.nlm.nih.gov/pubmed/28800133 http://dx.doi.org/10.1038/ismej.2017.136 |
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