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DISARM is a widespread bacterial defence system with broad anti-phage activities

The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction-modification and CRISPR-Cas systems. Here we report on a new defence system, DISARM (Defence Island System Associated with Re...

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Autores principales: Ofir, Gal, Melamed, Sarah, Sberro, Hila, Mukamel, Zohar, Silverman, Shahar, Yaakov, Gilad, Doron, Shany, Sorek, Rotem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739279/
https://www.ncbi.nlm.nih.gov/pubmed/29085076
http://dx.doi.org/10.1038/s41564-017-0051-0
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author Ofir, Gal
Melamed, Sarah
Sberro, Hila
Mukamel, Zohar
Silverman, Shahar
Yaakov, Gilad
Doron, Shany
Sorek, Rotem
author_facet Ofir, Gal
Melamed, Sarah
Sberro, Hila
Mukamel, Zohar
Silverman, Shahar
Yaakov, Gilad
Doron, Shany
Sorek, Rotem
author_sort Ofir, Gal
collection PubMed
description The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction-modification and CRISPR-Cas systems. Here we report on a new defence system, DISARM (Defence Island System Associated with Restriction-Modification), that is widespread in bacteria and archaea. DISARM is comprised of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase-D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945A DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all 3 major families of tailed double-stranded DNA phages. Using a series of gene deletions we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA, and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction-modification systems. Our results suggest that DISARM is a new type of multi-gene restriction-modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses.
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spelling pubmed-57392792018-04-30 DISARM is a widespread bacterial defence system with broad anti-phage activities Ofir, Gal Melamed, Sarah Sberro, Hila Mukamel, Zohar Silverman, Shahar Yaakov, Gilad Doron, Shany Sorek, Rotem Nat Microbiol Article The evolutionary pressure imposed by phage predation on bacteria and archaea has resulted in the development of effective anti-phage defence mechanisms, including restriction-modification and CRISPR-Cas systems. Here we report on a new defence system, DISARM (Defence Island System Associated with Restriction-Modification), that is widespread in bacteria and archaea. DISARM is comprised of five genes, including a DNA methylase and four other genes annotated as a helicase domain, a phospholipase-D (PLD) domain, a DUF1998 domain and a gene of unknown function. Engineering the Bacillus paralicheniformis 9945A DISARM system into Bacillus subtilis has rendered the engineered bacteria protected against phages from all 3 major families of tailed double-stranded DNA phages. Using a series of gene deletions we show that four of the five genes are essential for DISARM-mediated defence, with the fifth (PLD) being redundant for defence against some of the phages. We further show that DISARM restricts incoming phage DNA, and that the B. paralicheniformis DISARM methylase modifies host CCWGG motifs as a marker of self DNA akin to restriction-modification systems. Our results suggest that DISARM is a new type of multi-gene restriction-modification module, expanding the arsenal of defence systems known to be at the disposal of prokaryotes against their viruses. 2017-10-30 2018-01 /pmc/articles/PMC5739279/ /pubmed/29085076 http://dx.doi.org/10.1038/s41564-017-0051-0 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ofir, Gal
Melamed, Sarah
Sberro, Hila
Mukamel, Zohar
Silverman, Shahar
Yaakov, Gilad
Doron, Shany
Sorek, Rotem
DISARM is a widespread bacterial defence system with broad anti-phage activities
title DISARM is a widespread bacterial defence system with broad anti-phage activities
title_full DISARM is a widespread bacterial defence system with broad anti-phage activities
title_fullStr DISARM is a widespread bacterial defence system with broad anti-phage activities
title_full_unstemmed DISARM is a widespread bacterial defence system with broad anti-phage activities
title_short DISARM is a widespread bacterial defence system with broad anti-phage activities
title_sort disarm is a widespread bacterial defence system with broad anti-phage activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739279/
https://www.ncbi.nlm.nih.gov/pubmed/29085076
http://dx.doi.org/10.1038/s41564-017-0051-0
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