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Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction

Erectile dysfunction (ED) is a common complication of diabetes mellitus (DM). The exact role of the NGF/TrkA signaling pathway in the pathogenesis of diabetic ED is largely unknown. In the present study, we investigated the role of the NGF/TrkA signaling pathway in Sprague-Dawley rats with diabetic...

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Autores principales: Hou, Yi, Jia, Linpei, Zhang, Ying, Ji, Wei, Li, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739671/
https://www.ncbi.nlm.nih.gov/pubmed/29285284
http://dx.doi.org/10.18632/oncotarget.22389
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author Hou, Yi
Jia, Linpei
Zhang, Ying
Ji, Wei
Li, Hai
author_facet Hou, Yi
Jia, Linpei
Zhang, Ying
Ji, Wei
Li, Hai
author_sort Hou, Yi
collection PubMed
description Erectile dysfunction (ED) is a common complication of diabetes mellitus (DM). The exact role of the NGF/TrkA signaling pathway in the pathogenesis of diabetic ED is largely unknown. In the present study, we investigated the role of the NGF/TrkA signaling pathway in Sprague-Dawley rats with diabetic ED. Animals were divided into 2 groups: the normal group and the DM ED model group. The model group included the blank subgroup, the negative control (NC) subgroup, the TrkA subgroup and the TrkA + NGF subgroup. Erectile function, intracavernous pressure (ICP) and mean arterial pressure were measured respectively. Immunohistochemistry was used to examine the number of neuronal nitric oxide synthase (nNOS) expressing nerve fibers. The quantitative real-time polymerase chain reaction was applied to detect the mRNA expressions of NGF and TrkA in the cavernous tissue. Further, Western blotting was conducted to detect the expressions of NGF, TrkA and its downstream ERK pathway-related proteins. Higher erectile frequency, ICP values and diastolic function, more nNOS-positive nerve fibers, and decreased systolic function of the corpus cavernosum smooth muscle were found in the TrkA and TrkA+NGF groups when compared with the blank and the NC groups. Moreover, significantly higher mRNA expressions of NGF and TrkA, and upregulated protein expressions of NGF, TrkA, c-raf, ERK1/2 and CREB1 were found in the TrkA and the TrkA + NGF groups. In conclusion, downregulation in the NGF/TrkA signaling pathway may contribute to the pathogenesis of diabetic ED.
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spelling pubmed-57396712017-12-28 Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction Hou, Yi Jia, Linpei Zhang, Ying Ji, Wei Li, Hai Oncotarget Research Paper Erectile dysfunction (ED) is a common complication of diabetes mellitus (DM). The exact role of the NGF/TrkA signaling pathway in the pathogenesis of diabetic ED is largely unknown. In the present study, we investigated the role of the NGF/TrkA signaling pathway in Sprague-Dawley rats with diabetic ED. Animals were divided into 2 groups: the normal group and the DM ED model group. The model group included the blank subgroup, the negative control (NC) subgroup, the TrkA subgroup and the TrkA + NGF subgroup. Erectile function, intracavernous pressure (ICP) and mean arterial pressure were measured respectively. Immunohistochemistry was used to examine the number of neuronal nitric oxide synthase (nNOS) expressing nerve fibers. The quantitative real-time polymerase chain reaction was applied to detect the mRNA expressions of NGF and TrkA in the cavernous tissue. Further, Western blotting was conducted to detect the expressions of NGF, TrkA and its downstream ERK pathway-related proteins. Higher erectile frequency, ICP values and diastolic function, more nNOS-positive nerve fibers, and decreased systolic function of the corpus cavernosum smooth muscle were found in the TrkA and TrkA+NGF groups when compared with the blank and the NC groups. Moreover, significantly higher mRNA expressions of NGF and TrkA, and upregulated protein expressions of NGF, TrkA, c-raf, ERK1/2 and CREB1 were found in the TrkA and the TrkA + NGF groups. In conclusion, downregulation in the NGF/TrkA signaling pathway may contribute to the pathogenesis of diabetic ED. Impact Journals LLC 2017-11-11 /pmc/articles/PMC5739671/ /pubmed/29285284 http://dx.doi.org/10.18632/oncotarget.22389 Text en Copyright: © 2017 Hou et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Hou, Yi
Jia, Linpei
Zhang, Ying
Ji, Wei
Li, Hai
Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title_full Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title_fullStr Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title_full_unstemmed Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title_short Activation of the NGF/TrkA signaling pathway attenuates diabetic erectile dysfunction
title_sort activation of the ngf/trka signaling pathway attenuates diabetic erectile dysfunction
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739671/
https://www.ncbi.nlm.nih.gov/pubmed/29285284
http://dx.doi.org/10.18632/oncotarget.22389
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