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Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector

The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) has been investigated as a possible tool for gene therapy, but its inhibition by complement proteins in human serum limits its applicability. Here, we used the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) to con...

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Autores principales: Liu, Xingjian, Li, Yinü, Hu, Xiaoyuan, Yi, Yongzhu, Zhang, Zhifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739698/
https://www.ncbi.nlm.nih.gov/pubmed/29285311
http://dx.doi.org/10.18632/oncotarget.22522
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author Liu, Xingjian
Li, Yinü
Hu, Xiaoyuan
Yi, Yongzhu
Zhang, Zhifang
author_facet Liu, Xingjian
Li, Yinü
Hu, Xiaoyuan
Yi, Yongzhu
Zhang, Zhifang
author_sort Liu, Xingjian
collection PubMed
description The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) has been investigated as a possible tool for gene therapy, but its inhibition by complement proteins in human serum limits its applicability. Here, we used the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) to construct a gene delivery vector in which a reporter gene is driven by a cytomegalovirus IE promoter. Enhanced green fluorescent protein (EGFP) and luciferase reporter genes were used to test the efficiency of gene delivery. In vitro complement inactivation data showed that the recombinant BmNPV vector was more stable in human serum than the recombinant AcMNPV vector. The recombinant BmNPV vector successfully delivered the reporter genes into different tissues and organs in mice and chicks. These results demonstrate that the BmNPV vector is more stability against complement inactivation in human serum than the AcMNPV vector, and indicate that it may be useful as an effective gene delivery vector for gene therapy in vertebrates.
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spelling pubmed-57396982017-12-28 Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector Liu, Xingjian Li, Yinü Hu, Xiaoyuan Yi, Yongzhu Zhang, Zhifang Oncotarget Research Paper The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) has been investigated as a possible tool for gene therapy, but its inhibition by complement proteins in human serum limits its applicability. Here, we used the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) to construct a gene delivery vector in which a reporter gene is driven by a cytomegalovirus IE promoter. Enhanced green fluorescent protein (EGFP) and luciferase reporter genes were used to test the efficiency of gene delivery. In vitro complement inactivation data showed that the recombinant BmNPV vector was more stable in human serum than the recombinant AcMNPV vector. The recombinant BmNPV vector successfully delivered the reporter genes into different tissues and organs in mice and chicks. These results demonstrate that the BmNPV vector is more stability against complement inactivation in human serum than the AcMNPV vector, and indicate that it may be useful as an effective gene delivery vector for gene therapy in vertebrates. Impact Journals LLC 2017-11-20 /pmc/articles/PMC5739698/ /pubmed/29285311 http://dx.doi.org/10.18632/oncotarget.22522 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Liu, Xingjian
Li, Yinü
Hu, Xiaoyuan
Yi, Yongzhu
Zhang, Zhifang
Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title_full Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title_fullStr Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title_full_unstemmed Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title_short Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector
title_sort gene delivery and gene expression in vertebrate using baculovirus bombyx mori nucleopolyhedrovirus vector
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739698/
https://www.ncbi.nlm.nih.gov/pubmed/29285311
http://dx.doi.org/10.18632/oncotarget.22522
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