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CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells

Tumor-associated macrophages (TAMs) are involved in tumor progression and poor prognosis in several malignancies. We previously demonstrated the interaction between high numbers of infiltrating TAMs and poor prognosis in esophageal squamous cell carcinomas (ESCCs). To investigate the significance of...

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Autores principales: Hosono, Masayoshi, Koma, Yu-Ichiro, Takase, Nobuhisa, Urakawa, Naoki, Higashino, Nobuhide, Suemune, Kazuki, Kodaira, Himiko, Nishio, Mari, Shigeoka, Manabu, Kakeji, Yoshihiro, Yokozaki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739702/
https://www.ncbi.nlm.nih.gov/pubmed/29285315
http://dx.doi.org/10.18632/oncotarget.22526
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author Hosono, Masayoshi
Koma, Yu-Ichiro
Takase, Nobuhisa
Urakawa, Naoki
Higashino, Nobuhide
Suemune, Kazuki
Kodaira, Himiko
Nishio, Mari
Shigeoka, Manabu
Kakeji, Yoshihiro
Yokozaki, Hiroshi
author_facet Hosono, Masayoshi
Koma, Yu-Ichiro
Takase, Nobuhisa
Urakawa, Naoki
Higashino, Nobuhide
Suemune, Kazuki
Kodaira, Himiko
Nishio, Mari
Shigeoka, Manabu
Kakeji, Yoshihiro
Yokozaki, Hiroshi
author_sort Hosono, Masayoshi
collection PubMed
description Tumor-associated macrophages (TAMs) are involved in tumor progression and poor prognosis in several malignancies. We previously demonstrated the interaction between high numbers of infiltrating TAMs and poor prognosis in esophageal squamous cell carcinomas (ESCCs). To investigate the significance of TAMs in ESCC, we conducted a cDNA microarray analysis of peripheral blood monocytes (PBMo)-derived macrophages and PBMo-derived macrophages stimulated with conditioned media of TE-series ESCC cell lines (TAM-like PBMo-derived macrophages). C-X-C motif chemokine ligand 8 (CXCL8) was up-regulated in the TAM-like PBMo-derived macrophages. Here we confirmed a high expression level of CXCL8 in TAM-like PBMo-derived macrophages and the expression of CXCR1/2, known as CXCL8 receptors, in TE-series ESCC cell lines. Recombinant human CXCL8 induced the ESCC cell lines’ migration and invasion by the phosphorylation of Akt and Erk1/2. In indirect co-cultures, not only signal pathway inhibitors but also neutralizing antibodies against CXCL8, CXCR1 and CXCR2 suppressed these phenotypes induced by TAM-like PBMo-derived macrophages. Immunohistochemical analysis of 70 resected ESCC samples showed that high expression levels of CXCL8 in ESCC tissues were significantly associated with lymph node metastasis and poor prognosis. These results suggest that CXCL8 up-regulated in the microenvironment may contribute to ESCC progression by promoting cancer cells’ migration and invasion.
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spelling pubmed-57397022017-12-28 CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells Hosono, Masayoshi Koma, Yu-Ichiro Takase, Nobuhisa Urakawa, Naoki Higashino, Nobuhide Suemune, Kazuki Kodaira, Himiko Nishio, Mari Shigeoka, Manabu Kakeji, Yoshihiro Yokozaki, Hiroshi Oncotarget Research Paper Tumor-associated macrophages (TAMs) are involved in tumor progression and poor prognosis in several malignancies. We previously demonstrated the interaction between high numbers of infiltrating TAMs and poor prognosis in esophageal squamous cell carcinomas (ESCCs). To investigate the significance of TAMs in ESCC, we conducted a cDNA microarray analysis of peripheral blood monocytes (PBMo)-derived macrophages and PBMo-derived macrophages stimulated with conditioned media of TE-series ESCC cell lines (TAM-like PBMo-derived macrophages). C-X-C motif chemokine ligand 8 (CXCL8) was up-regulated in the TAM-like PBMo-derived macrophages. Here we confirmed a high expression level of CXCL8 in TAM-like PBMo-derived macrophages and the expression of CXCR1/2, known as CXCL8 receptors, in TE-series ESCC cell lines. Recombinant human CXCL8 induced the ESCC cell lines’ migration and invasion by the phosphorylation of Akt and Erk1/2. In indirect co-cultures, not only signal pathway inhibitors but also neutralizing antibodies against CXCL8, CXCR1 and CXCR2 suppressed these phenotypes induced by TAM-like PBMo-derived macrophages. Immunohistochemical analysis of 70 resected ESCC samples showed that high expression levels of CXCL8 in ESCC tissues were significantly associated with lymph node metastasis and poor prognosis. These results suggest that CXCL8 up-regulated in the microenvironment may contribute to ESCC progression by promoting cancer cells’ migration and invasion. Impact Journals LLC 2017-11-20 /pmc/articles/PMC5739702/ /pubmed/29285315 http://dx.doi.org/10.18632/oncotarget.22526 Text en Copyright: © 2017 Hosono et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Hosono, Masayoshi
Koma, Yu-Ichiro
Takase, Nobuhisa
Urakawa, Naoki
Higashino, Nobuhide
Suemune, Kazuki
Kodaira, Himiko
Nishio, Mari
Shigeoka, Manabu
Kakeji, Yoshihiro
Yokozaki, Hiroshi
CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title_full CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title_fullStr CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title_full_unstemmed CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title_short CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
title_sort cxcl8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739702/
https://www.ncbi.nlm.nih.gov/pubmed/29285315
http://dx.doi.org/10.18632/oncotarget.22526
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