Cargando…

Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios

There mainly exists four major myosin heavy chains (MyHC) (i.e., I, IIa, IIx, and IIb) in growing pigs. The current study aimed to explore the effects of low-protein diets supplemented with varying branched-chain amino acids (BCAAs) on muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in s...

Descripción completa

Detalles Bibliográficos
Autores principales: Duan, Yehui, Li, Fengna, Wang, Wenlong, Guo, Qiuping, Wen, Chaoyue, Yin, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739792/
https://www.ncbi.nlm.nih.gov/pubmed/29291007
http://dx.doi.org/10.18632/oncotarget.22205
_version_ 1783287940977262592
author Duan, Yehui
Li, Fengna
Wang, Wenlong
Guo, Qiuping
Wen, Chaoyue
Yin, Yulong
author_facet Duan, Yehui
Li, Fengna
Wang, Wenlong
Guo, Qiuping
Wen, Chaoyue
Yin, Yulong
author_sort Duan, Yehui
collection PubMed
description There mainly exists four major myosin heavy chains (MyHC) (i.e., I, IIa, IIx, and IIb) in growing pigs. The current study aimed to explore the effects of low-protein diets supplemented with varying branched-chain amino acids (BCAAs) on muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscles. Forty growing pigs (9.85 ± 0.35 kg) were allotted to 5 groups and fed with diets supplemented with varying leucine: isoleucine: valine ratios: 1:0.51:0.63 (20% crude protein, CP), 1:1:1 (17% CP), 1:0.75:0.75 (17% CP), 1:0.51:0.63 (17% CP), and 1:0.25:0.25 (17% CP), respectively. The skeletal muscles of different muscle fiber composition, that is, longissimus dorsi muscle (LM, a fast-twitch glycolytic muscle), biceps femoris muscle (BM, a mixed slow- and fast-twitch oxido-glycolytic muscle), and psoas major muscle (PM, a slow-twitch oxidative muscle) were collected and analyzed. Results showed that relative to the control group (1:0.51:0.63, 20% CP), the low-protein diets with the leucine: isoleucine: valine ratio ranging from 1:0.75:0.75 to 1:0.25:0.25 especially augmented the mRNA and protein abundance of MyHC I fibers in BM and lowered the mRNA abundance of MyHC IIb particularly in LM (P < 0.05), with a concurrent increase in the activation of AMPK and the mRNA abundance of SIRT and PGC-1α in BM (P < 0.05). The results reveal that low-protein diets supplemented with optimal BCAA ratio, i.e. 1:0.75:0.75-1:0.25:0.25, induce muscle more oxidative especially in oxido-glycolytic skeletal muscle of growing pigs. These effects are likely associated with the activation of the AMPK-SIRT1-PGC-1α axis.
format Online
Article
Text
id pubmed-5739792
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-57397922017-12-29 Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios Duan, Yehui Li, Fengna Wang, Wenlong Guo, Qiuping Wen, Chaoyue Yin, Yulong Oncotarget Research Paper There mainly exists four major myosin heavy chains (MyHC) (i.e., I, IIa, IIx, and IIb) in growing pigs. The current study aimed to explore the effects of low-protein diets supplemented with varying branched-chain amino acids (BCAAs) on muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscles. Forty growing pigs (9.85 ± 0.35 kg) were allotted to 5 groups and fed with diets supplemented with varying leucine: isoleucine: valine ratios: 1:0.51:0.63 (20% crude protein, CP), 1:1:1 (17% CP), 1:0.75:0.75 (17% CP), 1:0.51:0.63 (17% CP), and 1:0.25:0.25 (17% CP), respectively. The skeletal muscles of different muscle fiber composition, that is, longissimus dorsi muscle (LM, a fast-twitch glycolytic muscle), biceps femoris muscle (BM, a mixed slow- and fast-twitch oxido-glycolytic muscle), and psoas major muscle (PM, a slow-twitch oxidative muscle) were collected and analyzed. Results showed that relative to the control group (1:0.51:0.63, 20% CP), the low-protein diets with the leucine: isoleucine: valine ratio ranging from 1:0.75:0.75 to 1:0.25:0.25 especially augmented the mRNA and protein abundance of MyHC I fibers in BM and lowered the mRNA abundance of MyHC IIb particularly in LM (P < 0.05), with a concurrent increase in the activation of AMPK and the mRNA abundance of SIRT and PGC-1α in BM (P < 0.05). The results reveal that low-protein diets supplemented with optimal BCAA ratio, i.e. 1:0.75:0.75-1:0.25:0.25, induce muscle more oxidative especially in oxido-glycolytic skeletal muscle of growing pigs. These effects are likely associated with the activation of the AMPK-SIRT1-PGC-1α axis. Impact Journals LLC 2017-10-31 /pmc/articles/PMC5739792/ /pubmed/29291007 http://dx.doi.org/10.18632/oncotarget.22205 Text en Copyright: © 2017 Duan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Duan, Yehui
Li, Fengna
Wang, Wenlong
Guo, Qiuping
Wen, Chaoyue
Yin, Yulong
Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title_full Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title_fullStr Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title_full_unstemmed Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title_short Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
title_sort alteration of muscle fiber characteristics and the ampk-sirt1-pgc-1α axis in skeletal muscle of growing pigs fed low-protein diets with varying branched-chain amino acid ratios
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5739792/
https://www.ncbi.nlm.nih.gov/pubmed/29291007
http://dx.doi.org/10.18632/oncotarget.22205
work_keys_str_mv AT duanyehui alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios
AT lifengna alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios
AT wangwenlong alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios
AT guoqiuping alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios
AT wenchaoyue alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios
AT yinyulong alterationofmusclefibercharacteristicsandtheampksirt1pgc1aaxisinskeletalmuscleofgrowingpigsfedlowproteindietswithvaryingbranchedchainaminoacidratios