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Comparison of naturally aging and D-galactose induced aging model in beagle dogs

Animal models have been used to study aging for decades. In numerous aging studies, beagles are the most commonly used breed of dog. However, few studies have compared between naturally aging models and experimentally induced aging models in beagle dogs. In the present study, a D-galactose induced a...

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Autores principales: Ji, Musi, Su, Xiaohua, Liu, Jizhen, Zhao, Yi, Li, Zhidong, Xu, Xun, Li, Huawen, Nashun, Bayaer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740571/
https://www.ncbi.nlm.nih.gov/pubmed/29285136
http://dx.doi.org/10.3892/etm.2017.5327
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author Ji, Musi
Su, Xiaohua
Liu, Jizhen
Zhao, Yi
Li, Zhidong
Xu, Xun
Li, Huawen
Nashun, Bayaer
author_facet Ji, Musi
Su, Xiaohua
Liu, Jizhen
Zhao, Yi
Li, Zhidong
Xu, Xun
Li, Huawen
Nashun, Bayaer
author_sort Ji, Musi
collection PubMed
description Animal models have been used to study aging for decades. In numerous aging studies, beagles are the most commonly used breed of dog. However, few studies have compared between naturally aging models and experimentally induced aging models in beagle dogs. In the present study, a D-galactose induced aging model was compared with a naturally aging model, and young adult dogs were considered as the young control group. The level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum, and brain tissue were measured. Histopathological comparisons of the liver, kidneys, heart, lungs and spleen were evaluated using hematoxylin and eosin (H&E) staining, in addition, the brain was evaluated by H&E staining, and Nissl staining. The expression levels of aging-associated factors in the hippocampus, including proliferating cell nuclear antigen (PCNA), P16 and P21 were also determined through reverse transcription quantitative-polymerase chain reaction, and western blot analysis. The results indicated that D-galactose induced aging significantly increased the MDA level, while the levels of SOD and GSH-Px were diminished when compared with the young control group, which was similar to the naturally aging group. Parallel histopathological features were observed in the D-galactose induced aging and naturally aging groups compared with the young control group. However, a reduced expression level of PCNA, and increased expression levels of P16 and P21 were observed in the naturally ageing and induced aging groups compared with the young control group. The results of the current study demonstrated that the beagle dogs in D-galactose induced aging model exhibited significant similarities with the naturally aging model, providing evidence to support that the D-galactose induced aging model may be applied to aging studies.
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spelling pubmed-57405712017-12-28 Comparison of naturally aging and D-galactose induced aging model in beagle dogs Ji, Musi Su, Xiaohua Liu, Jizhen Zhao, Yi Li, Zhidong Xu, Xun Li, Huawen Nashun, Bayaer Exp Ther Med Articles Animal models have been used to study aging for decades. In numerous aging studies, beagles are the most commonly used breed of dog. However, few studies have compared between naturally aging models and experimentally induced aging models in beagle dogs. In the present study, a D-galactose induced aging model was compared with a naturally aging model, and young adult dogs were considered as the young control group. The level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum, and brain tissue were measured. Histopathological comparisons of the liver, kidneys, heart, lungs and spleen were evaluated using hematoxylin and eosin (H&E) staining, in addition, the brain was evaluated by H&E staining, and Nissl staining. The expression levels of aging-associated factors in the hippocampus, including proliferating cell nuclear antigen (PCNA), P16 and P21 were also determined through reverse transcription quantitative-polymerase chain reaction, and western blot analysis. The results indicated that D-galactose induced aging significantly increased the MDA level, while the levels of SOD and GSH-Px were diminished when compared with the young control group, which was similar to the naturally aging group. Parallel histopathological features were observed in the D-galactose induced aging and naturally aging groups compared with the young control group. However, a reduced expression level of PCNA, and increased expression levels of P16 and P21 were observed in the naturally ageing and induced aging groups compared with the young control group. The results of the current study demonstrated that the beagle dogs in D-galactose induced aging model exhibited significant similarities with the naturally aging model, providing evidence to support that the D-galactose induced aging model may be applied to aging studies. D.A. Spandidos 2017-12 2017-10-18 /pmc/articles/PMC5740571/ /pubmed/29285136 http://dx.doi.org/10.3892/etm.2017.5327 Text en Copyright: © Ji et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ji, Musi
Su, Xiaohua
Liu, Jizhen
Zhao, Yi
Li, Zhidong
Xu, Xun
Li, Huawen
Nashun, Bayaer
Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title_full Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title_fullStr Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title_full_unstemmed Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title_short Comparison of naturally aging and D-galactose induced aging model in beagle dogs
title_sort comparison of naturally aging and d-galactose induced aging model in beagle dogs
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740571/
https://www.ncbi.nlm.nih.gov/pubmed/29285136
http://dx.doi.org/10.3892/etm.2017.5327
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