Cargando…
Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer
Adoptive cellular immunotherapy (ACI) has been demonstrated to be a promising cancer therapeutic; however, the inefficient migration of adoptive immune cells to tumors is one of the rate-limiting factors of ACI. The present study investigated whether 2 Gy low dose irradiation (LDI) was able to incre...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740708/ https://www.ncbi.nlm.nih.gov/pubmed/29285113 http://dx.doi.org/10.3892/etm.2017.5305 |
_version_ | 1783288073524609024 |
---|---|
author | Du, Juan Su, Shu Li, Hongyan Shao, Jie Meng, Fanyan Yang, Mi Qian, Hanqing Zou, Zhengyun Qian, Xiaoping Liu, Baorui |
author_facet | Du, Juan Su, Shu Li, Hongyan Shao, Jie Meng, Fanyan Yang, Mi Qian, Hanqing Zou, Zhengyun Qian, Xiaoping Liu, Baorui |
author_sort | Du, Juan |
collection | PubMed |
description | Adoptive cellular immunotherapy (ACI) has been demonstrated to be a promising cancer therapeutic; however, the inefficient migration of adoptive immune cells to tumors is one of the rate-limiting factors of ACI. The present study investigated whether 2 Gy low dose irradiation (LDI) was able to increase the migration of adoptive lymphocytes to gastric cancer cells. Treatment with 2 Gy LDI resulted in marked chemokine (C-X-C motif) ligand 9 (CXCL9) and CXCL10 production from gastric cancer cell lines. A Transwell chamber migration assay demonstrated enhanced transmigration of cytotoxic T lymphocytes to gastric cancer cells following LDI treatment. After 2 Gy LDI application to established gastric carcinoma in nude mice, labeled immune cells were infused by intravenous injection and concentrated fluorescence signals were observed at the tumor sites within the mice, with a peak signal at 8-h LDI. Increased numbers of adoptive T cells at the tumor sites were also observed using flow cytometry. Furthermore, a case study of a patient with metastatic gastric cancer who had received ACI treatment combined with 2 Gy LDI provided further evidence that 2 Gy LDI is able to recruit antitumor effector T cells to tumor sites. Therefore, the ability of 2 Gy LDI to convert tumors into inflamed peripheral tissues may be exploited to overcome obstacles at the effector phase of the antitumor immune response and improve the therapeutic efficacy of immunotherapy. |
format | Online Article Text |
id | pubmed-5740708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-57407082017-12-28 Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer Du, Juan Su, Shu Li, Hongyan Shao, Jie Meng, Fanyan Yang, Mi Qian, Hanqing Zou, Zhengyun Qian, Xiaoping Liu, Baorui Exp Ther Med Articles Adoptive cellular immunotherapy (ACI) has been demonstrated to be a promising cancer therapeutic; however, the inefficient migration of adoptive immune cells to tumors is one of the rate-limiting factors of ACI. The present study investigated whether 2 Gy low dose irradiation (LDI) was able to increase the migration of adoptive lymphocytes to gastric cancer cells. Treatment with 2 Gy LDI resulted in marked chemokine (C-X-C motif) ligand 9 (CXCL9) and CXCL10 production from gastric cancer cell lines. A Transwell chamber migration assay demonstrated enhanced transmigration of cytotoxic T lymphocytes to gastric cancer cells following LDI treatment. After 2 Gy LDI application to established gastric carcinoma in nude mice, labeled immune cells were infused by intravenous injection and concentrated fluorescence signals were observed at the tumor sites within the mice, with a peak signal at 8-h LDI. Increased numbers of adoptive T cells at the tumor sites were also observed using flow cytometry. Furthermore, a case study of a patient with metastatic gastric cancer who had received ACI treatment combined with 2 Gy LDI provided further evidence that 2 Gy LDI is able to recruit antitumor effector T cells to tumor sites. Therefore, the ability of 2 Gy LDI to convert tumors into inflamed peripheral tissues may be exploited to overcome obstacles at the effector phase of the antitumor immune response and improve the therapeutic efficacy of immunotherapy. D.A. Spandidos 2017-12 2017-10-13 /pmc/articles/PMC5740708/ /pubmed/29285113 http://dx.doi.org/10.3892/etm.2017.5305 Text en Copyright: © Du et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Du, Juan Su, Shu Li, Hongyan Shao, Jie Meng, Fanyan Yang, Mi Qian, Hanqing Zou, Zhengyun Qian, Xiaoping Liu, Baorui Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title | Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title_full | Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title_fullStr | Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title_full_unstemmed | Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title_short | Low dose irradiation increases adoptive cytotoxic T lymphocyte migration in gastric cancer |
title_sort | low dose irradiation increases adoptive cytotoxic t lymphocyte migration in gastric cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740708/ https://www.ncbi.nlm.nih.gov/pubmed/29285113 http://dx.doi.org/10.3892/etm.2017.5305 |
work_keys_str_mv | AT dujuan lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT sushu lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT lihongyan lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT shaojie lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT mengfanyan lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT yangmi lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT qianhanqing lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT zouzhengyun lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT qianxiaoping lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer AT liubaorui lowdoseirradiationincreasesadoptivecytotoxictlymphocytemigrationingastriccancer |