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N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma
BACKGROUND: Because glycosylation is one of the most common post-translational modifications of proteins and because changes in glycosylation have been shown to have a significant correlation with the development of many cancer types, we investigated the serum N-glycome used to diagnose, stage and e...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740902/ https://www.ncbi.nlm.nih.gov/pubmed/29268706 http://dx.doi.org/10.1186/s12885-017-3891-3 |
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author | Chen, Jie Fang, Meng Chen, Xiaoling Yi, Changhong Ji, Jun Cheng, Cheng Wang, Mengmeng Gu, Xing Sun, Quansheng Gao, Chunfang |
author_facet | Chen, Jie Fang, Meng Chen, Xiaoling Yi, Changhong Ji, Jun Cheng, Cheng Wang, Mengmeng Gu, Xing Sun, Quansheng Gao, Chunfang |
author_sort | Chen, Jie |
collection | PubMed |
description | BACKGROUND: Because glycosylation is one of the most common post-translational modifications of proteins and because changes in glycosylation have been shown to have a significant correlation with the development of many cancer types, we investigated the serum N-glycome used to diagnose, stage and evaluate the pathological outcomes in IgD multiple myeloma. METHODS: Serum samples were available for 20 patients with IgD multiple myeloma, 41 patients with light chain multiple myeloma and 42 healthy control subjects. Serum N-glycans were released and analysed using DNA sequencer-assisted fluorophore-assisted capillary electrophoresis. RESULTS: Characteristic changes were revealed in the serum N-glycome of IgD myeloma. In particular, three N-glycans (NG1(6)A2F, Peak3; NG1(3)A2F, Peak4; NA2FB, Peak7) showed increased clinical value. The best area under the ROC curve of NG1(6)A2F to diagnose IgD myeloma was 0.981, with a 95.0% sensitivity and 95.2% specificity, and that of NG1(3)A2F was 0.936, with a 95.0% sensitivity and 78.6% specificity. The best area under the ROC curve of NA2FB/NG1(3)A2F to differentially diagnose IgD myeloma versus light chain myeloma was 0.744, with a 95.3% sensitivity and 50.0% specificity. The level of NG1(3)A2F was correlated with the international staging system, while the higher abundance of NA2FB presented in IgD myeloma was predictive of a shorter progression-free survival. CONCLUSIONS: The advent of serum N-glycan signatures may play a role in the diagnosis, staging and prognosis of IgD myeloma and will serve as the foundation for a precision medicine approach to this rare subtype of multiple myeloma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-017-3891-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5740902 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57409022018-01-03 N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma Chen, Jie Fang, Meng Chen, Xiaoling Yi, Changhong Ji, Jun Cheng, Cheng Wang, Mengmeng Gu, Xing Sun, Quansheng Gao, Chunfang BMC Cancer Research Article BACKGROUND: Because glycosylation is one of the most common post-translational modifications of proteins and because changes in glycosylation have been shown to have a significant correlation with the development of many cancer types, we investigated the serum N-glycome used to diagnose, stage and evaluate the pathological outcomes in IgD multiple myeloma. METHODS: Serum samples were available for 20 patients with IgD multiple myeloma, 41 patients with light chain multiple myeloma and 42 healthy control subjects. Serum N-glycans were released and analysed using DNA sequencer-assisted fluorophore-assisted capillary electrophoresis. RESULTS: Characteristic changes were revealed in the serum N-glycome of IgD myeloma. In particular, three N-glycans (NG1(6)A2F, Peak3; NG1(3)A2F, Peak4; NA2FB, Peak7) showed increased clinical value. The best area under the ROC curve of NG1(6)A2F to diagnose IgD myeloma was 0.981, with a 95.0% sensitivity and 95.2% specificity, and that of NG1(3)A2F was 0.936, with a 95.0% sensitivity and 78.6% specificity. The best area under the ROC curve of NA2FB/NG1(3)A2F to differentially diagnose IgD myeloma versus light chain myeloma was 0.744, with a 95.3% sensitivity and 50.0% specificity. The level of NG1(3)A2F was correlated with the international staging system, while the higher abundance of NA2FB presented in IgD myeloma was predictive of a shorter progression-free survival. CONCLUSIONS: The advent of serum N-glycan signatures may play a role in the diagnosis, staging and prognosis of IgD myeloma and will serve as the foundation for a precision medicine approach to this rare subtype of multiple myeloma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-017-3891-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-21 /pmc/articles/PMC5740902/ /pubmed/29268706 http://dx.doi.org/10.1186/s12885-017-3891-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chen, Jie Fang, Meng Chen, Xiaoling Yi, Changhong Ji, Jun Cheng, Cheng Wang, Mengmeng Gu, Xing Sun, Quansheng Gao, Chunfang N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title | N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title_full | N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title_fullStr | N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title_full_unstemmed | N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title_short | N-glycosylation of serum proteins for the assessment of patients with IgD multiple myeloma |
title_sort | n-glycosylation of serum proteins for the assessment of patients with igd multiple myeloma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740902/ https://www.ncbi.nlm.nih.gov/pubmed/29268706 http://dx.doi.org/10.1186/s12885-017-3891-3 |
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