Cargando…

An efficient transient expression system for gene function analysis in rose

BACKGROUND: Roses are widely used as garden ornamental plants and cut flowers. Rosa chinensis cv ‘Old Blush’ has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation syste...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lu, Jun, Bai, Mengjuan, Ren, Haoran, Liu, Jinyi, Wang, Changquan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740963/ https://www.ncbi.nlm.nih.gov/pubmed/29299050 http://dx.doi.org/10.1186/s13007-017-0268-1

Ejemplares similares

Poinsettia protoplasts - a simple, robust and efficient system for transient gene expression studies
por: Pitzschke, Andrea, et al.
Publicado: (2012)

Evolutionary Analysis and Functional Identification of Clock-Associated PSEUDO-RESPONSE REGULATOR (PRRs) Genes in the Flowering Regulation of Roses
por: Jalal, Abdul, et al.
Publicado: (2022)

AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings
por: Wu, Hung-Yi, et al.
Publicado: (2014)

A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes
por: Zhang, Yang, et al.
Publicado: (2011)

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
por: Zhang, Ke, et al.
Publicado: (2023)

A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.
por: Li, Yongpeng, et al.
Publicado: (2021)

An efficient system for Agrobacterium-mediated transient transformation in Pinus tabuliformis
por: Liu, Shuangwei, et al.
Publicado: (2020)

Efficient and inexpensive transient expression of multispecific multivalent antibodies in Expi293 cells
por: Fang, Xiaotian T., et al.
Publicado: (2017)

Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.
por: Fister, Andrew S., et al.
Publicado: (2016)

Alternate expression of CONSTANS-LIKE 4 in short days and CONSTANS in long days facilitates day-neutral response in Rosa chinensis
por: Lu, Jun, et al.
Publicado: (2020)

Methods for transient assay of gene function in floral tissues
por: Shang, Yongjin, et al.
Publicado: (2007)

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays
por: Poddar, Snigdha, et al.
Publicado: (2020)

Bacterial rose garden for metagenomic SNP-based phylogeny visualization
por: Alexeev, Dmitry, et al.
Publicado: (2015)

Rose long noncoding RNA lncWD83 promotes flowering by modulating ubiquitination of the floral repressor RcMYC2L
por: Yeqing, Chen, et al.
Publicado: (2023)

In planta transient expression as a system for genetic and biochemical analyses of chlorophyll biosynthesis
por: Sawers, Ruairidh JH, et al.
Publicado: (2006)

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)
por: Li, Haipeng, et al.
Publicado: (2018)

Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)
por: Wu, Jun-Zheng, et al.
Publicado: (2017)

An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar
por: Zheng, Lin, et al.
Publicado: (2021)

A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)
por: Juranić, Martina, et al.
Publicado: (2020)

A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides)
por: Takata, Naoki, et al.
Publicado: (2012)

Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes
por: Caisová, Lenka, et al.
Publicado: (2019)

The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species
por: Li, Jian-Feng, et al.
Publicado: (2009)

High-level diterpene production by transient expression in Nicotiana benthamiana
por: Brückner, Kathleen, et al.
Publicado: (2013)

KSN heterozygosity is associated with continuous flowering of Rosa rugosa Purple branch
por: Bai, Mengjuan, et al.
Publicado: (2021)

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean
por: Cheng, Yuanyuan, et al.
Publicado: (2021)

Framework for determining the optimal course of action when efficiency and affordability measures differ by perspective in cost-effectiveness analysis—with an illustrative case of HIV treatment in Mozambique
por: Corlis, Joseph, et al.
Publicado: (2023)

An efficient RNA interference screening strategy for gene functional analysis
por: Chang, Chih-Hung, et al.
Publicado: (2012)

The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells
por: Jiwaji, Meesbah, et al.
Publicado: (2010)

Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera
por: Li, Sufang, et al.
Publicado: (2022)

An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum
por: Wagner, Jeffrey C, et al.
Publicado: (2013)

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids
por: Ren, Rui, et al.
Publicado: (2020)

An efficient ensemble method for missing value imputation in microarray gene expression data
por: Zhu, Xinshan, et al.
Publicado: (2021)

A rapid and efficient method for uniform gene expression using the barley stripe mosaic virus
por: Cheuk, Arnaud, et al.
Publicado: (2017)

RoseNet: Rose leave dataset for the development of an automation system to recognize the diseases of rose
por: Sazzad, Sadia, et al.
Publicado: (2022)

A new design of a lentiviral shRNA vector with inducible co-expression of ARGONAUTE 2 for enhancing gene silencing efficiency
por: He, Jiening, et al.
Publicado: (2015)

Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry
por: Yan, Huiqing, et al.
Publicado: (2023)

A novel joint analysis framework improves identification of differentially expressed genes in cross disease transcriptomic analysis
por: Qin, Wenyi, et al.
Publicado: (2018)

MIKC(C)-type MADS-box genes in Rosa chinensis: the remarkable expansion of ABCDE model genes and their roles in floral organogenesis
por: Liu, Jinyi, et al.
Publicado: (2018)

GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system
por: Neuhold, Jana, et al.
Publicado: (2020)

Optimization of immune receptor-related hypersensitive cell death response assay using agrobacterium-mediated transient expression in tobacco plants
por: Huh, Sung Un
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_i3rpgreslrldr0hrl49vt0qpl5