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Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
To improve the antitumor efficacy of doxorubicin (DOX) and provide novel clinical treatment of gastric cancer, halloysite nanotubes (HNTs) loaded with DOX were encapsulated by soybean phospholipid (LIP) and the formed HNTs/DOX/LIP was systematically characterized via different techniques. The in vit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741065/ https://www.ncbi.nlm.nih.gov/pubmed/29296083 http://dx.doi.org/10.2147/IJN.S143928 |
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author | Li, Kai Zhang, Yongxing Chen, Mengting Hu, Yangyang Jiang, Weiliang Zhou, Li Li, Sisi Xu, Min Zhao, Qinghua Wan, Rong |
author_facet | Li, Kai Zhang, Yongxing Chen, Mengting Hu, Yangyang Jiang, Weiliang Zhou, Li Li, Sisi Xu, Min Zhao, Qinghua Wan, Rong |
author_sort | Li, Kai |
collection | PubMed |
description | To improve the antitumor efficacy of doxorubicin (DOX) and provide novel clinical treatment of gastric cancer, halloysite nanotubes (HNTs) loaded with DOX were encapsulated by soybean phospholipid (LIP) and the formed HNTs/DOX/LIP was systematically characterized via different techniques. The in vitro anticancer activity of HNTs/DOX/LIP was examined using an MTT assay. The antitumor efficacy and biocompatibility were monitored by measuring the tumor volume and assessing the blood routine and serum biochemistry using an ectopic implantation cancer model. The results show that when the concentration of HNTs was 3 mg/mL and the concentration of DOX was 1 mg/mL the optimal DOX loading efficiency was as high as 22.01%±0.43%. In vitro drug release behavior study demonstrated that HNTs/DOX/LIP shows a pH-responsive release property with fast drug release under acidic conditions (pH =5.4). MTT assays and in vivo experimental results revealed that HNTs/DOX/LIP exhibits a significantly higher inhibitory efficacy on the growth of mouse gastric cancer cells than free DOX at the same drug concentration. In addition, the life span of tumor-bearing mice in the HNTs/DOX/LIP-treated group was obviously prolonged compared with the control groups. Moreover, HNTs/DOX/LIP possessed excellent hemocompatibility as shown in the blood and histology studies. These findings indicated that the formed HNTs/DOX/LIP possesses higher antitumor efficacy and may be used as a targeted delivery nanoplatform for targeting therapy of different types of cancer cells. |
format | Online Article Text |
id | pubmed-5741065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-57410652018-01-02 Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes Li, Kai Zhang, Yongxing Chen, Mengting Hu, Yangyang Jiang, Weiliang Zhou, Li Li, Sisi Xu, Min Zhao, Qinghua Wan, Rong Int J Nanomedicine Original Research To improve the antitumor efficacy of doxorubicin (DOX) and provide novel clinical treatment of gastric cancer, halloysite nanotubes (HNTs) loaded with DOX were encapsulated by soybean phospholipid (LIP) and the formed HNTs/DOX/LIP was systematically characterized via different techniques. The in vitro anticancer activity of HNTs/DOX/LIP was examined using an MTT assay. The antitumor efficacy and biocompatibility were monitored by measuring the tumor volume and assessing the blood routine and serum biochemistry using an ectopic implantation cancer model. The results show that when the concentration of HNTs was 3 mg/mL and the concentration of DOX was 1 mg/mL the optimal DOX loading efficiency was as high as 22.01%±0.43%. In vitro drug release behavior study demonstrated that HNTs/DOX/LIP shows a pH-responsive release property with fast drug release under acidic conditions (pH =5.4). MTT assays and in vivo experimental results revealed that HNTs/DOX/LIP exhibits a significantly higher inhibitory efficacy on the growth of mouse gastric cancer cells than free DOX at the same drug concentration. In addition, the life span of tumor-bearing mice in the HNTs/DOX/LIP-treated group was obviously prolonged compared with the control groups. Moreover, HNTs/DOX/LIP possessed excellent hemocompatibility as shown in the blood and histology studies. These findings indicated that the formed HNTs/DOX/LIP possesses higher antitumor efficacy and may be used as a targeted delivery nanoplatform for targeting therapy of different types of cancer cells. Dove Medical Press 2017-12-19 /pmc/articles/PMC5741065/ /pubmed/29296083 http://dx.doi.org/10.2147/IJN.S143928 Text en © 2018 Li et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Li, Kai Zhang, Yongxing Chen, Mengting Hu, Yangyang Jiang, Weiliang Zhou, Li Li, Sisi Xu, Min Zhao, Qinghua Wan, Rong Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title | Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title_full | Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title_fullStr | Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title_full_unstemmed | Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title_short | Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
title_sort | enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741065/ https://www.ncbi.nlm.nih.gov/pubmed/29296083 http://dx.doi.org/10.2147/IJN.S143928 |
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