Cargando…

Structural constraints and enzymatic promiscuity in the Cas6-dependent generation of crRNAs

A hallmark of defense mechanisms based on clustered regularly interspaced short palindromic repeats (CRISPR) and associated sequences (Cas) are the crRNAs that guide these complexes in the destruction of invading DNA or RNA. Three separate CRISPR-Cas systems exist in the cyanobacterium Synechocystis...

Descripción completa

Detalles Bibliográficos
Autores principales:	Reimann, Viktoria, Alkhnbashi, Omer S., Saunders, Sita J., Scholz, Ingeborg, Hein, Stephanie, Backofen, Rolf, Hess, Wolfgang R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	RNA
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741207/ https://www.ncbi.nlm.nih.gov/pubmed/27599840 http://dx.doi.org/10.1093/nar/gkw786

Ejemplares similares

A Complex of Cas Proteins 5, 6, and 7 Is Required for the Biogenesis and Stability of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii
por: Brendel, Jutta, et al.
Publicado: (2014)

An Active Immune Defense with a Minimal CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) RNA and without the Cas6 Protein
por: Maier, Lisa-Katharina, et al.
Publicado: (2015)

CRISPR-Cas Systems in the Cyanobacterium Synechocystis sp. PCC6803 Exhibit Distinct Processing Pathways Involving at Least Two Cas6 and a Cmr2 Protein
por: Scholz, Ingeborg, et al.
Publicado: (2013)

CRISPRstrand: predicting repeat orientations to determine the crRNA-encoding strand at CRISPR loci
por: Alkhnbashi, Omer S., et al.
Publicado: (2014)

CRISPR-Cas-Docker: web-based in silico docking and machine learning-based classification of crRNAs with Cas proteins
por: Park, Ho-min, et al.
Publicado: (2023)

Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9
por: Jiao, Chunlei, et al.
Publicado: (2021)

PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
por: Mann, James G., et al.
Publicado: (2022)

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish
por: Kotani, Hirohito, et al.
Publicado: (2015)

Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length
por: Songailiene, Inga, et al.
Publicado: (2019)

CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA
por: Jedrzejczyk, D. J., et al.
Publicado: (2022)

CRISPRmap: an automated classification of repeat conservation in prokaryotic adaptive immune systems
por: Lange, Sita J., et al.
Publicado: (2013)

Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution
por: Armache, Jean-Paul, et al.
Publicado: (2013)

Comprehensive search for accessory proteins encoded with archaeal and bacterial type III CRISPR-cas gene cassettes reveals 39 new cas gene families
por: Shah, Shiraz A., et al.
Publicado: (2018)

Engineering CRISPR-Cpf1 crRNAs and mRNAs to maximize genome editing efficiency
por: Li, Bin, et al.
Publicado: (2017)

Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors
por: Liu, Yang, et al.
Publicado: (2022)

Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target
por: Leski, T. A., et al.
Publicado: (2023)

CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection
por: Mitrofanov, Alexander, et al.
Publicado: (2022)

Characterization of CRISPR RNA processing in Clostridium thermocellum and Methanococcus maripaludis
por: Richter, Hagen, et al.
Publicado: (2012)

HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells
por: Li, Qintong, et al.
Publicado: (2007)

Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system
por: Li, Yingnan, et al.
Publicado: (2023)

Casboundary: automated definition of integral Cas cassettes
por: Padilha, Victor A, et al.
Publicado: (2020)

CRISPRcasIdentifier: Machine learning for accurate identification and classification of CRISPR-Cas systems
por: Padilha, Victor A, et al.
Publicado: (2020)

An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains
por: Jäger, Dominik, et al.
Publicado: (2012)

Transcriptome-wide in vivo mapping of cleavage sites for the compact cyanobacterial ribonuclease E reveals insights into its function and substrate recognition
por: Hoffmann, Ute A, et al.
Publicado: (2021)

Horizontal gene transfer as a mechanism for the promiscuous acquisition of distinct classes of IRES by avian caliciviruses
por: Arhab, Yani, et al.
Publicado: (2021)

Molecular determinants for CRISPR RNA maturation in the Cas10–Csm complex and roles for non-Cas nucleases
por: Walker, Forrest C., et al.
Publicado: (2017)

The role of Cas8 in type I CRISPR interference
por: Cass, Simon D.B., et al.
Publicado: (2015)

Distance constraints between microRNA target sites dictate efficacy and cooperativity
por: Sætrom, Pål, et al.
Publicado: (2007)

Adaptation induced by self-targeting in a type I-B CRISPR-Cas system
por: Stachler, Aris-Edda, et al.
Publicado: (2020)

The CR3 motif of Rrp44p is important for interaction with the core exosome and exosome function
por: Schaeffer, Daneen, et al.
Publicado: (2012)

New insights into the fundamental role of topological constraints as a determinant of two-way junction conformation
por: Mustoe, Anthony M., et al.
Publicado: (2012)

Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12
por: Haque, Ariful, et al.
Publicado: (2010)

Topological constraints are major determinants of tRNA tertiary structure and dynamics and provide basis for tertiary folding cooperativity
por: Mustoe, Anthony M., et al.
Publicado: (2014)

S-Geranyl-2-thiouridine wobble nucleosides of bacterial tRNAs; chemical and enzymatic synthesis of S-geranylated-RNAs and their physicochemical characterization
por: Sierant, Malgorzata, et al.
Publicado: (2016)

Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs
por: Lee, Ho Joung, et al.
Publicado: (2022)

Spacer prioritization in CRISPR-Cas9 immunity is enabled by the leader RNA
por: Liao, Chunyu, et al.
Publicado: (2022)

JMJD6 and U2AF65 co-regulate alternative splicing in both JMJD6 enzymatic activity dependent and independent manner
por: Yi, Jia, et al.
Publicado: (2017)

A one-step platform for screening high-efficient and minimal off-target CRISPR/Cas13 crRNAs to eradicate SARS-CoV-2 virus for treatment of COVID-19 patients
por: Li, Pu-Yu, et al.
Publicado: (2022)

Gene Repression in Haloarchaea Using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas I-B System
por: Stachler, Aris-Edda, et al.
Publicado: (2016)

Current Bioinformatics Tools to Optimize CRISPR/Cas9 Experiments to Reduce Off-Target Effects
por: Naeem, Muhammad, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ssk2ld2lcul6o0472plrjb8flv