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MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/repe...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741829/ https://www.ncbi.nlm.nih.gov/pubmed/29187583 http://dx.doi.org/10.1042/BSR20171242 |
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author | Ou, Jianying Kou, Li Liang, Lingyan Tang, Chaogang |
author_facet | Ou, Jianying Kou, Li Liang, Lingyan Tang, Chaogang |
author_sort | Ou, Jianying |
collection | PubMed |
description | Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/reperfusion (I/R). Herein, our study showed that miR-375 expression was significantly down-regulated in the rat I/R brain. With the in vivo and in vitro I/R stroke models, we found that miR-375 mimic provides significant protection from injury to cerebral I/R, which is reflected by reduced infarct volumes and cell apoptosis, and increased proliferation and migration of PC12 cells. Mechanistically, our findings showed that miR-375 binds to 3′-UTR region of Ctgf mRNA, subsequently leading to the decreased expression of Ctgf in the I/R brain. Furthermore, we showed that miR-375/Ctgf-mediated protective effects are associated with p21/PI3K/Akt signaling pathways. Our findings thus provide a new insight into the mechanism of cerebral I/R injury and pave a potential new way for the therapy of cerebral I/R injury. |
format | Online Article Text |
id | pubmed-5741829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-57418292018-01-05 MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf Ou, Jianying Kou, Li Liang, Lingyan Tang, Chaogang Biosci Rep Research Articles Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/reperfusion (I/R). Herein, our study showed that miR-375 expression was significantly down-regulated in the rat I/R brain. With the in vivo and in vitro I/R stroke models, we found that miR-375 mimic provides significant protection from injury to cerebral I/R, which is reflected by reduced infarct volumes and cell apoptosis, and increased proliferation and migration of PC12 cells. Mechanistically, our findings showed that miR-375 binds to 3′-UTR region of Ctgf mRNA, subsequently leading to the decreased expression of Ctgf in the I/R brain. Furthermore, we showed that miR-375/Ctgf-mediated protective effects are associated with p21/PI3K/Akt signaling pathways. Our findings thus provide a new insight into the mechanism of cerebral I/R injury and pave a potential new way for the therapy of cerebral I/R injury. Portland Press Ltd. 2017-12-22 /pmc/articles/PMC5741829/ /pubmed/29187583 http://dx.doi.org/10.1042/BSR20171242 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Ou, Jianying Kou, Li Liang, Lingyan Tang, Chaogang MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title | MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title_full | MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title_fullStr | MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title_full_unstemmed | MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title_short | MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf |
title_sort | mir-375 attenuates injury of cerebral ischemia/reperfusion via targetting ctgf |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741829/ https://www.ncbi.nlm.nih.gov/pubmed/29187583 http://dx.doi.org/10.1042/BSR20171242 |
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