Cargando…

MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf

Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/repe...

Descripción completa

Detalles Bibliográficos
Autores principales: Ou, Jianying, Kou, Li, Liang, Lingyan, Tang, Chaogang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741829/
https://www.ncbi.nlm.nih.gov/pubmed/29187583
http://dx.doi.org/10.1042/BSR20171242
_version_ 1783288259927867392
author Ou, Jianying
Kou, Li
Liang, Lingyan
Tang, Chaogang
author_facet Ou, Jianying
Kou, Li
Liang, Lingyan
Tang, Chaogang
author_sort Ou, Jianying
collection PubMed
description Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/reperfusion (I/R). Herein, our study showed that miR-375 expression was significantly down-regulated in the rat I/R brain. With the in vivo and in vitro I/R stroke models, we found that miR-375 mimic provides significant protection from injury to cerebral I/R, which is reflected by reduced infarct volumes and cell apoptosis, and increased proliferation and migration of PC12 cells. Mechanistically, our findings showed that miR-375 binds to 3′-UTR region of Ctgf mRNA, subsequently leading to the decreased expression of Ctgf in the I/R brain. Furthermore, we showed that miR-375/Ctgf-mediated protective effects are associated with p21/PI3K/Akt signaling pathways. Our findings thus provide a new insight into the mechanism of cerebral I/R injury and pave a potential new way for the therapy of cerebral I/R injury.
format Online
Article
Text
id pubmed-5741829
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-57418292018-01-05 MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf Ou, Jianying Kou, Li Liang, Lingyan Tang, Chaogang Biosci Rep Research Articles Ischemic stroke is the leading cause of disability and deaths worldwide. MiRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/reperfusion (I/R). Herein, our study showed that miR-375 expression was significantly down-regulated in the rat I/R brain. With the in vivo and in vitro I/R stroke models, we found that miR-375 mimic provides significant protection from injury to cerebral I/R, which is reflected by reduced infarct volumes and cell apoptosis, and increased proliferation and migration of PC12 cells. Mechanistically, our findings showed that miR-375 binds to 3′-UTR region of Ctgf mRNA, subsequently leading to the decreased expression of Ctgf in the I/R brain. Furthermore, we showed that miR-375/Ctgf-mediated protective effects are associated with p21/PI3K/Akt signaling pathways. Our findings thus provide a new insight into the mechanism of cerebral I/R injury and pave a potential new way for the therapy of cerebral I/R injury. Portland Press Ltd. 2017-12-22 /pmc/articles/PMC5741829/ /pubmed/29187583 http://dx.doi.org/10.1042/BSR20171242 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Ou, Jianying
Kou, Li
Liang, Lingyan
Tang, Chaogang
MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title_full MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title_fullStr MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title_full_unstemmed MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title_short MiR-375 attenuates injury of cerebral ischemia/reperfusion via targetting Ctgf
title_sort mir-375 attenuates injury of cerebral ischemia/reperfusion via targetting ctgf
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741829/
https://www.ncbi.nlm.nih.gov/pubmed/29187583
http://dx.doi.org/10.1042/BSR20171242
work_keys_str_mv AT oujianying mir375attenuatesinjuryofcerebralischemiareperfusionviatargettingctgf
AT kouli mir375attenuatesinjuryofcerebralischemiareperfusionviatargettingctgf
AT lianglingyan mir375attenuatesinjuryofcerebralischemiareperfusionviatargettingctgf
AT tangchaogang mir375attenuatesinjuryofcerebralischemiareperfusionviatargettingctgf