Cargando…

At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana

Chlorophylls and carotenoids not only function in photosynthesis and photoprotection but are also involved in the assembly of thylakoid membranes and the stabilization of apoproteins in photosystems. In this study, we identified a nuclear gene required for chlorophyll and carotenoid metabolism, name...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Xing-Qi, Zhao, Lei, Rui, Jin-Di, Zhou, Chang-Fang, Zhuang, Zhong, Lu, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742152/
https://www.ncbi.nlm.nih.gov/pubmed/29312395
http://dx.doi.org/10.3389/fpls.2017.02140
_version_ 1783288318661754880
author Huang, Xing-Qi
Zhao, Lei
Rui, Jin-Di
Zhou, Chang-Fang
Zhuang, Zhong
Lu, Shan
author_facet Huang, Xing-Qi
Zhao, Lei
Rui, Jin-Di
Zhou, Chang-Fang
Zhuang, Zhong
Lu, Shan
author_sort Huang, Xing-Qi
collection PubMed
description Chlorophylls and carotenoids not only function in photosynthesis and photoprotection but are also involved in the assembly of thylakoid membranes and the stabilization of apoproteins in photosystems. In this study, we identified a nuclear gene required for chlorophyll and carotenoid metabolism, namely, DWARF AND YELLOW 1 (DY1). Growth of the loss-of-function dy1 mutant was severely retarded, and the seedlings of this mutant accumulated significantly less amounts of both chlorophylls and carotenoids in cotyledons and rosette leaves, although genes related to pigment metabolism did not show corresponding fluctuation at the transcriptional level. In chloroplasts of the dy1 leaves, thylakoids were loosely packed into grana. The dy1 mutant also possessed severely impaired photosynthetic and photoprotective abilities. DY1 encodes a chloroplast stroma protein that is highly conserved in vascular plants. Our results demonstrated that after the full-length DY1 (53 kDa) was imported into the chloroplast and its N-terminal transit peptide was processed, the C-terminal end of this premature DY1 (42 kDa) was also removed during the maturation of rosette leaves, resulting in a 24-kDa mature peptide. Our blue native PAGE and Western blot analyses showed the presence of both premature and mature forms of DY1 in protein complexes. The involvement of DY1 in chloroplast development is discussed.
format Online
Article
Text
id pubmed-5742152
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-57421522018-01-08 At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana Huang, Xing-Qi Zhao, Lei Rui, Jin-Di Zhou, Chang-Fang Zhuang, Zhong Lu, Shan Front Plant Sci Plant Science Chlorophylls and carotenoids not only function in photosynthesis and photoprotection but are also involved in the assembly of thylakoid membranes and the stabilization of apoproteins in photosystems. In this study, we identified a nuclear gene required for chlorophyll and carotenoid metabolism, namely, DWARF AND YELLOW 1 (DY1). Growth of the loss-of-function dy1 mutant was severely retarded, and the seedlings of this mutant accumulated significantly less amounts of both chlorophylls and carotenoids in cotyledons and rosette leaves, although genes related to pigment metabolism did not show corresponding fluctuation at the transcriptional level. In chloroplasts of the dy1 leaves, thylakoids were loosely packed into grana. The dy1 mutant also possessed severely impaired photosynthetic and photoprotective abilities. DY1 encodes a chloroplast stroma protein that is highly conserved in vascular plants. Our results demonstrated that after the full-length DY1 (53 kDa) was imported into the chloroplast and its N-terminal transit peptide was processed, the C-terminal end of this premature DY1 (42 kDa) was also removed during the maturation of rosette leaves, resulting in a 24-kDa mature peptide. Our blue native PAGE and Western blot analyses showed the presence of both premature and mature forms of DY1 in protein complexes. The involvement of DY1 in chloroplast development is discussed. Frontiers Media S.A. 2017-12-19 /pmc/articles/PMC5742152/ /pubmed/29312395 http://dx.doi.org/10.3389/fpls.2017.02140 Text en Copyright © 2017 Huang, Zhao, Rui, Zhou, Zhuang and Lu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Huang, Xing-Qi
Zhao, Lei
Rui, Jin-Di
Zhou, Chang-Fang
Zhuang, Zhong
Lu, Shan
At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title_full At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title_fullStr At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title_full_unstemmed At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title_short At5g19540 Encodes a Novel Protein That Affects Pigment Metabolism and Chloroplast Development in Arabidopsis thaliana
title_sort at5g19540 encodes a novel protein that affects pigment metabolism and chloroplast development in arabidopsis thaliana
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5742152/
https://www.ncbi.nlm.nih.gov/pubmed/29312395
http://dx.doi.org/10.3389/fpls.2017.02140
work_keys_str_mv AT huangxingqi at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana
AT zhaolei at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana
AT ruijindi at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana
AT zhouchangfang at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana
AT zhuangzhong at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana
AT lushan at5g19540encodesanovelproteinthataffectspigmentmetabolismandchloroplastdevelopmentinarabidopsisthaliana