Influence of Maternal Care on Behavioural Development of Domestic Dogs (Canis Familiaris) Living in a Home Environment

SIMPLE SUMMARY: Maternal care in dogs and its impact on the behavioural development of puppies has become the subject of growing research interest. In order to determine the effects of maternal care on the behaviour of family dog puppies, maternal care during the first three weeks after birth was observed in 12 litters of puppies reared in a home environment (72 puppies). The behavioural responses of the puppies were assessed at two months of age, in an arena and an isolation test. In both tests, the amount of maternal care was found to be positively associated with some stress behaviours and, in the arena test, also with the puppies’ interest in an unfamiliar person who was present during the test. These behaviours are similar to those observed during the separation of other young mammals from an attachment figure with which they have a high quality of bond. Amount of maternal care was also found to be negatively associated with exploration and play. The difference in results between the present study and our previous study involving laboratory dogs reared in relative social isolation suggests that the developmental trajectory of puppies is influenced by a combination of maternal behaviour and social and physical environmental enrichment. ABSTRACT: Maternal care has been shown to affect the development of the brain, behaviour, social skills and emotional systems of the young of many mammalian species including dogs. The aim of the present study was to determine the effects of maternal care on the behavioural responses of family dog puppies towards environmental and social stimuli. In order to do this, maternal care (licking puppy’s ano-genital area, licking the puppy, nursing and mother-puppy contact) during the first three weeks after birth was assessed in 12 litters of domestic dog puppies reared in home environments (total = 72 puppies). The behavioural responses of puppies were assessed in an arena and an isolation test, which were performed when the puppies were two-month old. Data were analysed using principal components analysis and projection to latent structures regression. A systematic relationship was found between maternal care and behaviour in both tests. In the arena test, maternal care was found to be positively associated with approach to the stranger, attention oriented to the stranger, time spent near the enclosure, yawning, whining and yelping (R(2)Y = 0.613, p = 8.2 × 10(−9)). Amount of maternal care was negatively associated with the number of squares crossed and the time spent individually playing with the rope. In the isolation test, the amount of maternal care was positively associated with standing posture, paw lifting, and howling, and it was negatively associated with yawning, lying down and nose licking (R(2)Y = 0.507, p = 0.000626). These results suggest that the amount of maternal care received during early life influences the pattern of behavioural responses and coping strategies of puppies at two-months of age. On the basis of these findings it could be speculated that early maternal care contributes to adaption to the environment in which family puppies are developing, with particular regard to social relationships with people.