Cargando…
Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells
MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles i...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743841/ https://www.ncbi.nlm.nih.gov/pubmed/29291166 http://dx.doi.org/10.7150/ntno.22960 |
_version_ | 1783288637567270912 |
---|---|
author | Li, Jing Huang, Jin Yang, Xiaohai Yang, Yanjing Quan, Ke Xie, Nuli Wu, Yanan Ma, Changbei Wang, Kemin |
author_facet | Li, Jing Huang, Jin Yang, Xiaohai Yang, Yanjing Quan, Ke Xie, Nuli Wu, Yanan Ma, Changbei Wang, Kemin |
author_sort | Li, Jing |
collection | PubMed |
description | MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed “flares”. In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully. Compared to the traditional single-color-based nanoflares, the two-color-based nanoflares could offer more reliable and practical information for cancer detection, improving the accuracy of early disease diagnosis. |
format | Online Article Text |
id | pubmed-5743841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-57438412018-01-01 Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells Li, Jing Huang, Jin Yang, Xiaohai Yang, Yanjing Quan, Ke Xie, Nuli Wu, Yanan Ma, Changbei Wang, Kemin Nanotheranostics Research Paper MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed “flares”. In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully. Compared to the traditional single-color-based nanoflares, the two-color-based nanoflares could offer more reliable and practical information for cancer detection, improving the accuracy of early disease diagnosis. Ivyspring International Publisher 2018-01-01 /pmc/articles/PMC5743841/ /pubmed/29291166 http://dx.doi.org/10.7150/ntno.22960 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Jing Huang, Jin Yang, Xiaohai Yang, Yanjing Quan, Ke Xie, Nuli Wu, Yanan Ma, Changbei Wang, Kemin Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title | Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title_full | Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title_fullStr | Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title_full_unstemmed | Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title_short | Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells |
title_sort | two-color-based nanoflares for multiplexed micrornas imaging in live cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743841/ https://www.ncbi.nlm.nih.gov/pubmed/29291166 http://dx.doi.org/10.7150/ntno.22960 |
work_keys_str_mv | AT lijing twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT huangjin twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT yangxiaohai twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT yangyanjing twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT quanke twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT xienuli twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT wuyanan twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT machangbei twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells AT wangkemin twocolorbasednanoflaresformultiplexedmicrornasimaginginlivecells |