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Comprehensive functional genomics using Caenorhabditis elegans as a model organism

We have been working on functional genomics using C. elegans as a model organism. We first used cell-type specific markers and preexisting mutants to investigate how genotype-phenotype causal relationships are regulated. With the aid of transgenic methods, we analyzed various biological processes in...

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Detalles Bibliográficos
Autor principal: MITANI, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japan Academy 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743858/
https://www.ncbi.nlm.nih.gov/pubmed/29021508
http://dx.doi.org/10.2183/pjab.93.036
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author MITANI, Shohei
author_facet MITANI, Shohei
author_sort MITANI, Shohei
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description We have been working on functional genomics using C. elegans as a model organism. We first used cell-type specific markers and preexisting mutants to investigate how genotype-phenotype causal relationships are regulated. With the aid of transgenic methods, we analyzed various biological processes in C. elegans. We have developed efficient methods to isolate gene knockout strains. Thousands of strains isolated this way are used by many researchers and have revealed many biological mechanisms. We have also developed methods to examine the functions of genes in a comprehensive manner by integrating transgenes into chromosomes, designing conditional knockouts, and creating balancers for lethal mutations. A combination of these biological resources and techniques will be useful to understand the functions of genes in C. elegans, which has many genes that are orthologous to those of higher organisms including humans.
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spelling pubmed-57438582018-01-04 Comprehensive functional genomics using Caenorhabditis elegans as a model organism MITANI, Shohei Proc Jpn Acad Ser B Phys Biol Sci Review We have been working on functional genomics using C. elegans as a model organism. We first used cell-type specific markers and preexisting mutants to investigate how genotype-phenotype causal relationships are regulated. With the aid of transgenic methods, we analyzed various biological processes in C. elegans. We have developed efficient methods to isolate gene knockout strains. Thousands of strains isolated this way are used by many researchers and have revealed many biological mechanisms. We have also developed methods to examine the functions of genes in a comprehensive manner by integrating transgenes into chromosomes, designing conditional knockouts, and creating balancers for lethal mutations. A combination of these biological resources and techniques will be useful to understand the functions of genes in C. elegans, which has many genes that are orthologous to those of higher organisms including humans. The Japan Academy 2017-10-11 /pmc/articles/PMC5743858/ /pubmed/29021508 http://dx.doi.org/10.2183/pjab.93.036 Text en © 2017 The Japan Academy This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
MITANI, Shohei
Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title_full Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title_fullStr Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title_full_unstemmed Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title_short Comprehensive functional genomics using Caenorhabditis elegans as a model organism
title_sort comprehensive functional genomics using caenorhabditis elegans as a model organism
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743858/
https://www.ncbi.nlm.nih.gov/pubmed/29021508
http://dx.doi.org/10.2183/pjab.93.036
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