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Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids

In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (...

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Autores principales: Kizziah, James L., Manning, Keith A., Dearborn, Altaira D., Wall, Erin A., Klenow, Laura, Hill, Rosanne L. L., Spilman, Michael S., Stagg, Scott M., Christie, Gail E., Dokland, Terje
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744158/
https://www.ncbi.nlm.nih.gov/pubmed/29258203
http://dx.doi.org/10.3390/v9120384
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author Kizziah, James L.
Manning, Keith A.
Dearborn, Altaira D.
Wall, Erin A.
Klenow, Laura
Hill, Rosanne L. L.
Spilman, Michael S.
Stagg, Scott M.
Christie, Gail E.
Dokland, Terje
author_facet Kizziah, James L.
Manning, Keith A.
Dearborn, Altaira D.
Wall, Erin A.
Klenow, Laura
Hill, Rosanne L. L.
Spilman, Michael S.
Stagg, Scott M.
Christie, Gail E.
Dokland, Terje
author_sort Kizziah, James L.
collection PubMed
description In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. Staphylococcus aureus bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called S. aureus pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80α to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80α are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80α mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80α and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80α and SaPI1 capsids.
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spelling pubmed-57441582017-12-31 Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids Kizziah, James L. Manning, Keith A. Dearborn, Altaira D. Wall, Erin A. Klenow, Laura Hill, Rosanne L. L. Spilman, Michael S. Stagg, Scott M. Christie, Gail E. Dokland, Terje Viruses Article In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other capsid-associated proteins, including the scaffolding protein (SP) that serves as a chaperone for the assembly process. Staphylococcus aureus bacteriophage 80α is capable of high frequency mobilization of mobile genetic elements called S. aureus pathogenicity islands (SaPIs), such as SaPI1. SaPI1 redirects the assembly pathway of 80α to form capsids that are smaller than those normally made by the phage alone. Both CP and SP of 80α are N-terminally processed by a host-encoded protease, Prp. We have analyzed phage mutants that express pre-cleaved or uncleavable versions of CP or SP, and show that the N-terminal sequence in SP is absolutely required for assembly, but does not need to be cleaved in order to produce viable capsids. Mutants with pre-cleaved or uncleavable CP display normal viability. We have used cryo-EM to solve the structures of mature capsids from an 80α mutant expressing uncleavable CP, and from wildtype SaPI1. Comparisons with structures of 80α and SaPI1 procapsids show that capsid maturation involves major conformational changes in CP, consistent with a release of the CP N-arm by SP. The hexamers reorganize during maturation to accommodate the different environments in the 80α and SaPI1 capsids. MDPI 2017-12-16 /pmc/articles/PMC5744158/ /pubmed/29258203 http://dx.doi.org/10.3390/v9120384 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kizziah, James L.
Manning, Keith A.
Dearborn, Altaira D.
Wall, Erin A.
Klenow, Laura
Hill, Rosanne L. L.
Spilman, Michael S.
Stagg, Scott M.
Christie, Gail E.
Dokland, Terje
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title_full Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title_fullStr Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title_full_unstemmed Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title_short Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
title_sort cleavage and structural transitions during maturation of staphylococcus aureus bacteriophage 80α and sapi1 capsids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744158/
https://www.ncbi.nlm.nih.gov/pubmed/29258203
http://dx.doi.org/10.3390/v9120384
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