Cargando…

The impact of persistent bacterial bronchitis on the pulmonary microbiome of children

INTRODUCTION: Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected br...

Descripción completa

Detalles Bibliográficos
Autores principales: Cuthbertson, Leah, Craven, Vanessa, Bingle, Lynne, Cookson, William O. C. M., Everard, Mark L., Moffatt, Miriam F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744971/
https://www.ncbi.nlm.nih.gov/pubmed/29281698
http://dx.doi.org/10.1371/journal.pone.0190075
_version_ 1783288845431734272
author Cuthbertson, Leah
Craven, Vanessa
Bingle, Lynne
Cookson, William O. C. M.
Everard, Mark L.
Moffatt, Miriam F.
author_facet Cuthbertson, Leah
Craven, Vanessa
Bingle, Lynne
Cookson, William O. C. M.
Everard, Mark L.
Moffatt, Miriam F.
author_sort Cuthbertson, Leah
collection PubMed
description INTRODUCTION: Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. RESULTS: No significant difference in bacterial diversity or community composition (R(2) = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R(2) = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9%) cases the dominant organism by sequencing was not identified by standard routine clinical culture. CONCLUSION: The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden.
format Online
Article
Text
id pubmed-5744971
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-57449712018-01-09 The impact of persistent bacterial bronchitis on the pulmonary microbiome of children Cuthbertson, Leah Craven, Vanessa Bingle, Lynne Cookson, William O. C. M. Everard, Mark L. Moffatt, Miriam F. PLoS One Research Article INTRODUCTION: Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. RESULTS: No significant difference in bacterial diversity or community composition (R(2) = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R(2) = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9%) cases the dominant organism by sequencing was not identified by standard routine clinical culture. CONCLUSION: The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden. Public Library of Science 2017-12-27 /pmc/articles/PMC5744971/ /pubmed/29281698 http://dx.doi.org/10.1371/journal.pone.0190075 Text en © 2017 Cuthbertson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cuthbertson, Leah
Craven, Vanessa
Bingle, Lynne
Cookson, William O. C. M.
Everard, Mark L.
Moffatt, Miriam F.
The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title_full The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title_fullStr The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title_full_unstemmed The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title_short The impact of persistent bacterial bronchitis on the pulmonary microbiome of children
title_sort impact of persistent bacterial bronchitis on the pulmonary microbiome of children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744971/
https://www.ncbi.nlm.nih.gov/pubmed/29281698
http://dx.doi.org/10.1371/journal.pone.0190075
work_keys_str_mv AT cuthbertsonleah theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT cravenvanessa theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT binglelynne theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT cooksonwilliamocm theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT everardmarkl theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT moffattmiriamf theimpactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT cuthbertsonleah impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT cravenvanessa impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT binglelynne impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT cooksonwilliamocm impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT everardmarkl impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren
AT moffattmiriamf impactofpersistentbacterialbronchitisonthepulmonarymicrobiomeofchildren