Library Construction from Subnanogram DNA for Pelagic Sea Water and Deep-Sea Sediments

Shotgun metagenomics is a low biased technology for assessing environmental microbial diversity and function. However, the requirement for a sufficient amount of DNA and the contamination of inhibitors in environmental DNA leads to difficulties in constructing a shotgun metagenomic library. We herei...

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Detalles Bibliográficos
Autores principales: Hirai, Miho, Nishi, Shinro, Tsuda, Miwako, Sunamura, Michinari, Takaki, Yoshihiro, Nunoura, Takuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2017
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745018/
https://www.ncbi.nlm.nih.gov/pubmed/29187708
http://dx.doi.org/10.1264/jsme2.ME17132

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