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Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy

Okayama University-type retinal prosthesis (OUReP(TM)) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coup...

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Autores principales: MATSUO, Toshihiko, UCHIDA, Tetsuya, NITTA, Makoto, YAMASHITA, Koichiro, TAKEI, Shigiko, IDO, Daisuke, TANAKA, Mamoru, OGUCHI, Masao, FURUKAWA, Toshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745168/
https://www.ncbi.nlm.nih.gov/pubmed/29046507
http://dx.doi.org/10.1292/jvms.17-0450
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author MATSUO, Toshihiko
UCHIDA, Tetsuya
NITTA, Makoto
YAMASHITA, Koichiro
TAKEI, Shigiko
IDO, Daisuke
TANAKA, Mamoru
OGUCHI, Masao
FURUKAWA, Toshinori
author_facet MATSUO, Toshihiko
UCHIDA, Tetsuya
NITTA, Makoto
YAMASHITA, Koichiro
TAKEI, Shigiko
IDO, Daisuke
TANAKA, Mamoru
OGUCHI, Masao
FURUKAWA, Toshinori
author_sort MATSUO, Toshihiko
collection PubMed
description Okayama University-type retinal prosthesis (OUReP(TM)) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery. The surface electric potential of the removed dye-coupled films in response to light was measured by the Kelvin Probe system. At surgery, rolled-up dye-coupled films in 5 × 5 mm square size could be inserted into subretinal space of retinal detachment induced by fluid injection with a 38-gauge polyimide tip. Retinal attachment was maintained by silicone oil injection in vitreous cavity. At autopsy, the retina in all dogs maintained the ganglion cell layer, inner and outer nuclear layers while it lost the outer segments in some part. All 5 sheets of removed dye-coupled films maintained the dye color. One sheet of the 5-month implanted film showed proportional increase of surface potential in response to increasing light intensity. Subretinal implantation of OUReP(TM) by vitrectomy was technically feasible in canine eyes, and OUReP(TM) maintained the function of generating light-evoked surface potential after 5 months in subretinal implantation.
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spelling pubmed-57451682018-01-05 Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy MATSUO, Toshihiko UCHIDA, Tetsuya NITTA, Makoto YAMASHITA, Koichiro TAKEI, Shigiko IDO, Daisuke TANAKA, Mamoru OGUCHI, Masao FURUKAWA, Toshinori J Vet Med Sci Surgery Okayama University-type retinal prosthesis (OUReP(TM)) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility of subretinal implantation and functional durability of dye-coupled films in the subretinal space. The dye-coupled films were implanted subretinally by 25-gauge vitrectomy in the right eye of 11 normal beagle dogs: 2 dogs served for film removal after 5-month film implantation, 3 dogs for film removal after 3-month film implantation, 3 dogs for 3-month film implantation and pathological examination, and 3 dogs for sham surgery. The surface electric potential of the removed dye-coupled films in response to light was measured by the Kelvin Probe system. At surgery, rolled-up dye-coupled films in 5 × 5 mm square size could be inserted into subretinal space of retinal detachment induced by fluid injection with a 38-gauge polyimide tip. Retinal attachment was maintained by silicone oil injection in vitreous cavity. At autopsy, the retina in all dogs maintained the ganglion cell layer, inner and outer nuclear layers while it lost the outer segments in some part. All 5 sheets of removed dye-coupled films maintained the dye color. One sheet of the 5-month implanted film showed proportional increase of surface potential in response to increasing light intensity. Subretinal implantation of OUReP(TM) by vitrectomy was technically feasible in canine eyes, and OUReP(TM) maintained the function of generating light-evoked surface potential after 5 months in subretinal implantation. The Japanese Society of Veterinary Science 2017-10-18 2017-12 /pmc/articles/PMC5745168/ /pubmed/29046507 http://dx.doi.org/10.1292/jvms.17-0450 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Surgery
MATSUO, Toshihiko
UCHIDA, Tetsuya
NITTA, Makoto
YAMASHITA, Koichiro
TAKEI, Shigiko
IDO, Daisuke
TANAKA, Mamoru
OGUCHI, Masao
FURUKAWA, Toshinori
Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title_full Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title_fullStr Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title_full_unstemmed Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title_short Subretinal implantation of Okayama University-type retinal prosthesis (OUReP(TM)) in canine eyes by vitrectomy
title_sort subretinal implantation of okayama university-type retinal prosthesis (ourep(tm)) in canine eyes by vitrectomy
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745168/
https://www.ncbi.nlm.nih.gov/pubmed/29046507
http://dx.doi.org/10.1292/jvms.17-0450
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