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Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets

The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first mor...

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Autores principales: TAKAHASHI, Naoki, TANGKAWATTANA, Prasarn, OOTOMO, Yoshiki, HIROSE, Takuya, MINAGUCHI, Jun, UEDA, Hiromi, YAMADA, Michi, TAKEHANA, Kazushige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745172/
https://www.ncbi.nlm.nih.gov/pubmed/29070765
http://dx.doi.org/10.1292/jvms.17-0436
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author TAKAHASHI, Naoki
TANGKAWATTANA, Prasarn
OOTOMO, Yoshiki
HIROSE, Takuya
MINAGUCHI, Jun
UEDA, Hiromi
YAMADA, Michi
TAKEHANA, Kazushige
author_facet TAKAHASHI, Naoki
TANGKAWATTANA, Prasarn
OOTOMO, Yoshiki
HIROSE, Takuya
MINAGUCHI, Jun
UEDA, Hiromi
YAMADA, Michi
TAKEHANA, Kazushige
author_sort TAKAHASHI, Naoki
collection PubMed
description The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P<0.05) were found in almost all morphometric aspects among the age groups, except for the number of Cp/cell (P=0.545) and thickness of the Cp (P=0.105). A decrease of cell density corresponded with an increase in the length of the Cp, which were extended to connect either with the Cp of the other tenocytes or the surrounding endotendineum. Moreover, an increase of the fascicle area reflected the increase in tendon diameter. The revealed morphometric characteristics are thus the outcome of tendon growth.
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spelling pubmed-57451722018-01-05 Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets TAKAHASHI, Naoki TANGKAWATTANA, Prasarn OOTOMO, Yoshiki HIROSE, Takuya MINAGUCHI, Jun UEDA, Hiromi YAMADA, Michi TAKEHANA, Kazushige J Vet Med Sci Anatomy The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P<0.05) were found in almost all morphometric aspects among the age groups, except for the number of Cp/cell (P=0.545) and thickness of the Cp (P=0.105). A decrease of cell density corresponded with an increase in the length of the Cp, which were extended to connect either with the Cp of the other tenocytes or the surrounding endotendineum. Moreover, an increase of the fascicle area reflected the increase in tendon diameter. The revealed morphometric characteristics are thus the outcome of tendon growth. The Japanese Society of Veterinary Science 2017-10-25 2017-12 /pmc/articles/PMC5745172/ /pubmed/29070765 http://dx.doi.org/10.1292/jvms.17-0436 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Anatomy
TAKAHASHI, Naoki
TANGKAWATTANA, Prasarn
OOTOMO, Yoshiki
HIROSE, Takuya
MINAGUCHI, Jun
UEDA, Hiromi
YAMADA, Michi
TAKEHANA, Kazushige
Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title_full Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title_fullStr Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title_full_unstemmed Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title_short Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
title_sort morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
topic Anatomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745172/
https://www.ncbi.nlm.nih.gov/pubmed/29070765
http://dx.doi.org/10.1292/jvms.17-0436
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