Cargando…

Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods

Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramatla, Tsepo, Ngoma, Lubanza, Adetunji, Modupeade, Mwanza, Mulunda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745477/
https://www.ncbi.nlm.nih.gov/pubmed/29215578
http://dx.doi.org/10.3390/antibiotics6040034
_version_ 1783288911450079232
author Ramatla, Tsepo
Ngoma, Lubanza
Adetunji, Modupeade
Mwanza, Mulunda
author_facet Ramatla, Tsepo
Ngoma, Lubanza
Adetunji, Modupeade
Mwanza, Mulunda
author_sort Ramatla, Tsepo
collection PubMed
description Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat from sales points were analysed for ciprofloxacin, streptomycin, tetracycline, and sulphanilamide residues. Overall, ELISA analysis showed that 56, 34, 18, and 25.3% of the samples tested positive for ciprofloxacin, streptomycin, sulphanilamide and tetracycline residues respectively while TLC and HPLC detected 21.4, 29.4, 92.5, and 14.6%, and 8.3, 41.1, 88.8, and 14.6% of the samples as containing the residues, with ciprofloxacin and sulphanilamide having the lowest and highest occurrence, respectively. Furthermore, the concentrations of antibiotic residues were in the ranges of 19.8–92.8, 26.6–489.1, 14.2–1280.8, and 42.6–355.6 μg/kg with ELISA, while HPLC detected concentration ranges of 20.7–82.1, 41.8–320.8, 65.2–952.2 and 32.8–95.6 μg/kg for sulphanilamide, tetracycline, streptomycin, and ciprofloxacin, respectively. Mean ciprofloxacin and streptomycin residue levels were above the Codex/SA MRL recommended limit, while 3% of the samples contained multidrug residues. Although some of the mean residues levels were below the permissible limits, the co-occurrence of multidrug residues in some of the samples calls for concern.
format Online
Article
Text
id pubmed-5745477
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-57454772018-01-02 Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods Ramatla, Tsepo Ngoma, Lubanza Adetunji, Modupeade Mwanza, Mulunda Antibiotics (Basel) Article Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat from sales points were analysed for ciprofloxacin, streptomycin, tetracycline, and sulphanilamide residues. Overall, ELISA analysis showed that 56, 34, 18, and 25.3% of the samples tested positive for ciprofloxacin, streptomycin, sulphanilamide and tetracycline residues respectively while TLC and HPLC detected 21.4, 29.4, 92.5, and 14.6%, and 8.3, 41.1, 88.8, and 14.6% of the samples as containing the residues, with ciprofloxacin and sulphanilamide having the lowest and highest occurrence, respectively. Furthermore, the concentrations of antibiotic residues were in the ranges of 19.8–92.8, 26.6–489.1, 14.2–1280.8, and 42.6–355.6 μg/kg with ELISA, while HPLC detected concentration ranges of 20.7–82.1, 41.8–320.8, 65.2–952.2 and 32.8–95.6 μg/kg for sulphanilamide, tetracycline, streptomycin, and ciprofloxacin, respectively. Mean ciprofloxacin and streptomycin residue levels were above the Codex/SA MRL recommended limit, while 3% of the samples contained multidrug residues. Although some of the mean residues levels were below the permissible limits, the co-occurrence of multidrug residues in some of the samples calls for concern. MDPI 2017-12-07 /pmc/articles/PMC5745477/ /pubmed/29215578 http://dx.doi.org/10.3390/antibiotics6040034 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramatla, Tsepo
Ngoma, Lubanza
Adetunji, Modupeade
Mwanza, Mulunda
Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title_full Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title_fullStr Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title_full_unstemmed Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title_short Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods
title_sort evaluation of antibiotic residues in raw meat using different analytical methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745477/
https://www.ncbi.nlm.nih.gov/pubmed/29215578
http://dx.doi.org/10.3390/antibiotics6040034
work_keys_str_mv AT ramatlatsepo evaluationofantibioticresiduesinrawmeatusingdifferentanalyticalmethods
AT ngomalubanza evaluationofantibioticresiduesinrawmeatusingdifferentanalyticalmethods
AT adetunjimodupeade evaluationofantibioticresiduesinrawmeatusingdifferentanalyticalmethods
AT mwanzamulunda evaluationofantibioticresiduesinrawmeatusingdifferentanalyticalmethods