Cargando…
Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745671/ https://www.ncbi.nlm.nih.gov/pubmed/29387727 http://dx.doi.org/10.1155/2017/9342714 |
_version_ | 1783288949285847040 |
---|---|
author | Hu, Lei Gao, Zhenhua Xu, Junji Zhu, Zhao Fan, Zhipeng Zhang, Chunmei Wang, Jinsong Wang, Songlin |
author_facet | Hu, Lei Gao, Zhenhua Xu, Junji Zhu, Zhao Fan, Zhipeng Zhang, Chunmei Wang, Jinsong Wang, Songlin |
author_sort | Hu, Lei |
collection | PubMed |
description | Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases. |
format | Online Article Text |
id | pubmed-5745671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-57456712018-01-31 Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration Hu, Lei Gao, Zhenhua Xu, Junji Zhu, Zhao Fan, Zhipeng Zhang, Chunmei Wang, Jinsong Wang, Songlin Biomed Res Int Research Article Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases. Hindawi 2017 2017-12-13 /pmc/articles/PMC5745671/ /pubmed/29387727 http://dx.doi.org/10.1155/2017/9342714 Text en Copyright © 2017 Lei Hu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hu, Lei Gao, Zhenhua Xu, Junji Zhu, Zhao Fan, Zhipeng Zhang, Chunmei Wang, Jinsong Wang, Songlin Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title | Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title_full | Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title_fullStr | Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title_full_unstemmed | Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title_short | Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration |
title_sort | decellularized swine dental pulp as a bioscaffold for pulp regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745671/ https://www.ncbi.nlm.nih.gov/pubmed/29387727 http://dx.doi.org/10.1155/2017/9342714 |
work_keys_str_mv | AT hulei decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT gaozhenhua decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT xujunji decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT zhuzhao decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT fanzhipeng decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT zhangchunmei decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT wangjinsong decellularizedswinedentalpulpasabioscaffoldforpulpregeneration AT wangsonglin decellularizedswinedentalpulpasabioscaffoldforpulpregeneration |