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Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration

Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are...

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Autores principales: Hu, Lei, Gao, Zhenhua, Xu, Junji, Zhu, Zhao, Fan, Zhipeng, Zhang, Chunmei, Wang, Jinsong, Wang, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745671/
https://www.ncbi.nlm.nih.gov/pubmed/29387727
http://dx.doi.org/10.1155/2017/9342714
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author Hu, Lei
Gao, Zhenhua
Xu, Junji
Zhu, Zhao
Fan, Zhipeng
Zhang, Chunmei
Wang, Jinsong
Wang, Songlin
author_facet Hu, Lei
Gao, Zhenhua
Xu, Junji
Zhu, Zhao
Fan, Zhipeng
Zhang, Chunmei
Wang, Jinsong
Wang, Songlin
author_sort Hu, Lei
collection PubMed
description Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases.
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spelling pubmed-57456712018-01-31 Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration Hu, Lei Gao, Zhenhua Xu, Junji Zhu, Zhao Fan, Zhipeng Zhang, Chunmei Wang, Jinsong Wang, Songlin Biomed Res Int Research Article Endodontic regeneration shows promise in treating dental pulp diseases; however, no suitable scaffolds exist for pulp regeneration. Acellular natural extracellular matrix (ECM) is a favorable scaffold for tissue regeneration since the anatomical structure and ECM of the natural tissues or organs are well-preserved. Xenogeneic ECM is superior to autologous or allogeneic ECM in tissue engineering for its unlimited resources. This study investigated the characteristics of decellularized dental pulp ECM from swine and evaluated whether it could mediate pulp regeneration. Dental pulps were acquired from the mandible anterior teeth of swine 12 months of age and decellularized with 10% sodium dodecyl sulfate (SDS) combined with Triton X-100. Pulp regeneration was conducted by seeding human dental pulp stem cells into decellularized pulp and transplanted subcutaneously into nude mice for 8 weeks. The decellularized pulp demonstrated preserved natural shape and structure without any cellular components. Histological analysis showed excellent ECM preservation and pulp-like tissue, and newly formed mineralized tissues were regenerated after being transplanted in vivo. In conclusion, decellularized swine dental pulp maintains ECM components favoring stem cell proliferation and differentiation, thus representing a suitable scaffold for improving clinical outcomes and functions of teeth with dental pulp diseases. Hindawi 2017 2017-12-13 /pmc/articles/PMC5745671/ /pubmed/29387727 http://dx.doi.org/10.1155/2017/9342714 Text en Copyright © 2017 Lei Hu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hu, Lei
Gao, Zhenhua
Xu, Junji
Zhu, Zhao
Fan, Zhipeng
Zhang, Chunmei
Wang, Jinsong
Wang, Songlin
Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title_full Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title_fullStr Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title_full_unstemmed Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title_short Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration
title_sort decellularized swine dental pulp as a bioscaffold for pulp regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745671/
https://www.ncbi.nlm.nih.gov/pubmed/29387727
http://dx.doi.org/10.1155/2017/9342714
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