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Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression
BACKGROUND: In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the deg...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745754/ https://www.ncbi.nlm.nih.gov/pubmed/29282148 http://dx.doi.org/10.1186/s13071-017-2547-0 |
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author | Ng, Sook-Luan Nordin, Anisah Abd Ghafar, Norzana Suboh, Yusof Ab Rahim, Noraina Chua, Kien-Hui |
author_facet | Ng, Sook-Luan Nordin, Anisah Abd Ghafar, Norzana Suboh, Yusof Ab Rahim, Noraina Chua, Kien-Hui |
author_sort | Ng, Sook-Luan |
collection | PubMed |
description | BACKGROUND: In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba. RESULTS: In this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect. CONCLUSIONS: All ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s13071-017-2547-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5745754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57457542018-01-03 Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression Ng, Sook-Luan Nordin, Anisah Abd Ghafar, Norzana Suboh, Yusof Ab Rahim, Noraina Chua, Kien-Hui Parasit Vectors Research BACKGROUND: In recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba. RESULTS: In this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect. CONCLUSIONS: All ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s13071-017-2547-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-28 /pmc/articles/PMC5745754/ /pubmed/29282148 http://dx.doi.org/10.1186/s13071-017-2547-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ng, Sook-Luan Nordin, Anisah Abd Ghafar, Norzana Suboh, Yusof Ab Rahim, Noraina Chua, Kien-Hui Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title | Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title_full | Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title_fullStr | Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title_full_unstemmed | Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title_short | Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression |
title_sort | acanthamoeba-mediated cytopathic effect correlates with mbp and ahlbp mrna expression |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745754/ https://www.ncbi.nlm.nih.gov/pubmed/29282148 http://dx.doi.org/10.1186/s13071-017-2547-0 |
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