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Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material
Pancreatic ductal adenocarcinoma (PDAC) continues to carry the lowest survival rates among all solid tumors. A marked resistance against available therapies, late clinical presentation and insufficient means for early diagnosis contribute to the dismal prognosis. Novel biomarkers are thus required t...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746138/ https://www.ncbi.nlm.nih.gov/pubmed/29296236 http://dx.doi.org/10.18632/oncotarget.22601 |
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author | Gress, Thomas M. Lausser, Ludwig Schirra, Lyn-Rouven Ortmüller, Lisa Diels, Ramona Kong, Bo Michalski, Christoph W. Hackert, Thilo Strobel, Oliver Giese, Nathalia A. Schenk, Miriam Lawlor, Rita T. Scarpa, Aldo Kestler, Hans A. Buchholz, Malte |
author_facet | Gress, Thomas M. Lausser, Ludwig Schirra, Lyn-Rouven Ortmüller, Lisa Diels, Ramona Kong, Bo Michalski, Christoph W. Hackert, Thilo Strobel, Oliver Giese, Nathalia A. Schenk, Miriam Lawlor, Rita T. Scarpa, Aldo Kestler, Hans A. Buchholz, Malte |
author_sort | Gress, Thomas M. |
collection | PubMed |
description | Pancreatic ductal adenocarcinoma (PDAC) continues to carry the lowest survival rates among all solid tumors. A marked resistance against available therapies, late clinical presentation and insufficient means for early diagnosis contribute to the dismal prognosis. Novel biomarkers are thus required to aid treatment decisions and improve patient outcomes. We describe here a multi-omics molecular platform that allows for the first time to simultaneously analyze miRNA and mRNA expression patterns from minimal amounts of biopsy material on a single microfluidic TaqMan Array card. Expression profiles were generated from 113 prospectively collected fine needle aspiration biopsies (FNAB) from patients undergoing surgery for suspect masses in the pancreas. Molecular classifiers were constructed using support vector machines, and rigorously evaluated for diagnostic performance using 10×10fold cross validation. The final combined miRNA/mRNA classifier demonstrated a sensitivity of 91.7%, a specificity of 94.5%, and an overall diagnostic accuracy of 93.0% for the differentiation between PDAC and benign pancreatic masses, clearly outperfoming miRNA-only classifiers. The classification algorithm also performed very well in the diagnosis of other types of solid tumors (acinar cell carcinomas, ampullary cancer and distal bile duct carcinomas), but was less suited for the diagnostic analysis of cystic lesions. We thus demonstrate that simultaneous analysis of miRNA and mRNA biomarkers from FNAB samples using multi-omics TaqMan Array cards is suitable to differentiate suspect solid pancreatic masses with high precision. |
format | Online Article Text |
id | pubmed-5746138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57461382018-01-02 Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material Gress, Thomas M. Lausser, Ludwig Schirra, Lyn-Rouven Ortmüller, Lisa Diels, Ramona Kong, Bo Michalski, Christoph W. Hackert, Thilo Strobel, Oliver Giese, Nathalia A. Schenk, Miriam Lawlor, Rita T. Scarpa, Aldo Kestler, Hans A. Buchholz, Malte Oncotarget Research Paper Pancreatic ductal adenocarcinoma (PDAC) continues to carry the lowest survival rates among all solid tumors. A marked resistance against available therapies, late clinical presentation and insufficient means for early diagnosis contribute to the dismal prognosis. Novel biomarkers are thus required to aid treatment decisions and improve patient outcomes. We describe here a multi-omics molecular platform that allows for the first time to simultaneously analyze miRNA and mRNA expression patterns from minimal amounts of biopsy material on a single microfluidic TaqMan Array card. Expression profiles were generated from 113 prospectively collected fine needle aspiration biopsies (FNAB) from patients undergoing surgery for suspect masses in the pancreas. Molecular classifiers were constructed using support vector machines, and rigorously evaluated for diagnostic performance using 10×10fold cross validation. The final combined miRNA/mRNA classifier demonstrated a sensitivity of 91.7%, a specificity of 94.5%, and an overall diagnostic accuracy of 93.0% for the differentiation between PDAC and benign pancreatic masses, clearly outperfoming miRNA-only classifiers. The classification algorithm also performed very well in the diagnosis of other types of solid tumors (acinar cell carcinomas, ampullary cancer and distal bile duct carcinomas), but was less suited for the diagnostic analysis of cystic lesions. We thus demonstrate that simultaneous analysis of miRNA and mRNA biomarkers from FNAB samples using multi-omics TaqMan Array cards is suitable to differentiate suspect solid pancreatic masses with high precision. Impact Journals LLC 2017-11-21 /pmc/articles/PMC5746138/ /pubmed/29296236 http://dx.doi.org/10.18632/oncotarget.22601 Text en Copyright: © 2017 Gress et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Gress, Thomas M. Lausser, Ludwig Schirra, Lyn-Rouven Ortmüller, Lisa Diels, Ramona Kong, Bo Michalski, Christoph W. Hackert, Thilo Strobel, Oliver Giese, Nathalia A. Schenk, Miriam Lawlor, Rita T. Scarpa, Aldo Kestler, Hans A. Buchholz, Malte Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title | Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title_full | Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title_fullStr | Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title_full_unstemmed | Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title_short | Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
title_sort | combined microrna and mrna microfluidic taqman array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746138/ https://www.ncbi.nlm.nih.gov/pubmed/29296236 http://dx.doi.org/10.18632/oncotarget.22601 |
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