CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma

In this study, we explored the function and mechanism of CDKN2B genes in verapamil (VER)-induced reversal of resistance to doxorubicin (ADM) chemotherapy in hepatocellular carcinoma (HCC). We examined 4 HCC cell lines and found that the expression levels of CDKN2B genes correlated with the level of...

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Autores principales: Zhang, Tengyue, Ma, Kelong, Huang, Jin, Wang, Shitang, Liu, Yabei, Fan, Gaofei, Liu, Miao, Yang, Guangshan, Wang, Cheng, Fan, Pingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746364/
https://www.ncbi.nlm.nih.gov/pubmed/29299129
http://dx.doi.org/10.18632/oncotarget.22123
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author Zhang, Tengyue
Ma, Kelong
Huang, Jin
Wang, Shitang
Liu, Yabei
Fan, Gaofei
Liu, Miao
Yang, Guangshan
Wang, Cheng
Fan, Pingsheng
author_facet Zhang, Tengyue
Ma, Kelong
Huang, Jin
Wang, Shitang
Liu, Yabei
Fan, Gaofei
Liu, Miao
Yang, Guangshan
Wang, Cheng
Fan, Pingsheng
author_sort Zhang, Tengyue
collection PubMed
description In this study, we explored the function and mechanism of CDKN2B genes in verapamil (VER)-induced reversal of resistance to doxorubicin (ADM) chemotherapy in hepatocellular carcinoma (HCC). We examined 4 HCC cell lines and found that the expression levels of CDKN2B genes correlated with the level of apoptosis induced by ADM+VER. Overexpression of CDKN2B genes promoted apoptosis in cells treated with VER+ADM. CDKN2B knockdown using siRNA weakened the effect of ADM+VER, indicating that ADM+VER promotes HCC cell apoptosis and that CDKN2B genes participate in VER-mediated promotion in tumor cell apoptosis. Future research will further explore the functional mechanism, and the associated signal transduction pathways via which CDKN2B affects HCC drug resistance.
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spelling pubmed-57463642018-01-03 CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma Zhang, Tengyue Ma, Kelong Huang, Jin Wang, Shitang Liu, Yabei Fan, Gaofei Liu, Miao Yang, Guangshan Wang, Cheng Fan, Pingsheng Oncotarget Research Paper In this study, we explored the function and mechanism of CDKN2B genes in verapamil (VER)-induced reversal of resistance to doxorubicin (ADM) chemotherapy in hepatocellular carcinoma (HCC). We examined 4 HCC cell lines and found that the expression levels of CDKN2B genes correlated with the level of apoptosis induced by ADM+VER. Overexpression of CDKN2B genes promoted apoptosis in cells treated with VER+ADM. CDKN2B knockdown using siRNA weakened the effect of ADM+VER, indicating that ADM+VER promotes HCC cell apoptosis and that CDKN2B genes participate in VER-mediated promotion in tumor cell apoptosis. Future research will further explore the functional mechanism, and the associated signal transduction pathways via which CDKN2B affects HCC drug resistance. Impact Journals LLC 2017-10-26 /pmc/articles/PMC5746364/ /pubmed/29299129 http://dx.doi.org/10.18632/oncotarget.22123 Text en Copyright: © 2017 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Zhang, Tengyue
Ma, Kelong
Huang, Jin
Wang, Shitang
Liu, Yabei
Fan, Gaofei
Liu, Miao
Yang, Guangshan
Wang, Cheng
Fan, Pingsheng
CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title_full CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title_fullStr CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title_full_unstemmed CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title_short CDKN2B is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
title_sort cdkn2b is critical for verapamil-mediated reversal of doxorubicin resistance in hepatocellular carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746364/
https://www.ncbi.nlm.nih.gov/pubmed/29299129
http://dx.doi.org/10.18632/oncotarget.22123
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