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Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the norm...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746404/ https://www.ncbi.nlm.nih.gov/pubmed/29299169 http://dx.doi.org/10.18632/oncotarget.22821 |
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author | Liu, HX Xu, MQ Li, SP Tian, S Guo, MX Qi, JY He, CJ Zhao, XS |
author_facet | Liu, HX Xu, MQ Li, SP Tian, S Guo, MX Qi, JY He, CJ Zhao, XS |
author_sort | Liu, HX |
collection | PubMed |
description | Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the normal hepatocytes. JLGTE activated AMP-activated protein kinase (AMPK) signaling, which was required for its cytotoxicity against hepatocellular carcinoma cells. Silence of AMPKα1, via targeted short hairpin RNAs or CRISPR-Cas9 genome editing, inhibited JLGTE-induced AMPK activation and HepG2 cell apoptosis. Further, in-activation of AMPK by a dominant negative AMPKα1 (T172A) also alleviated JLGTE's cytotoxicity against HepG2 cells. On the other hand, forced-activation of AMPK by introduction of a constitutively-active AMPKα1 (T172D) mimicked JLGTE's actions and led to HepG2 cell apoptosis. These results suggest that JLGTE inhibits human hepatocellular carcinoma cells possibly via activating AMPK. |
format | Online Article Text |
id | pubmed-5746404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-57464042018-01-03 Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK Liu, HX Xu, MQ Li, SP Tian, S Guo, MX Qi, JY He, CJ Zhao, XS Oncotarget Research Paper Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the normal hepatocytes. JLGTE activated AMP-activated protein kinase (AMPK) signaling, which was required for its cytotoxicity against hepatocellular carcinoma cells. Silence of AMPKα1, via targeted short hairpin RNAs or CRISPR-Cas9 genome editing, inhibited JLGTE-induced AMPK activation and HepG2 cell apoptosis. Further, in-activation of AMPK by a dominant negative AMPKα1 (T172A) also alleviated JLGTE's cytotoxicity against HepG2 cells. On the other hand, forced-activation of AMPK by introduction of a constitutively-active AMPKα1 (T172D) mimicked JLGTE's actions and led to HepG2 cell apoptosis. These results suggest that JLGTE inhibits human hepatocellular carcinoma cells possibly via activating AMPK. Impact Journals LLC 2017-11-30 /pmc/articles/PMC5746404/ /pubmed/29299169 http://dx.doi.org/10.18632/oncotarget.22821 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Liu, HX Xu, MQ Li, SP Tian, S Guo, MX Qi, JY He, CJ Zhao, XS Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title | Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title_full | Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title_fullStr | Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title_full_unstemmed | Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title_short | Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK |
title_sort | jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating ampk |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746404/ https://www.ncbi.nlm.nih.gov/pubmed/29299169 http://dx.doi.org/10.18632/oncotarget.22821 |
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