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Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK

Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the norm...

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Autores principales: Liu, HX, Xu, MQ, Li, SP, Tian, S, Guo, MX, Qi, JY, He, CJ, Zhao, XS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746404/
https://www.ncbi.nlm.nih.gov/pubmed/29299169
http://dx.doi.org/10.18632/oncotarget.22821
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author Liu, HX
Xu, MQ
Li, SP
Tian, S
Guo, MX
Qi, JY
He, CJ
Zhao, XS
author_facet Liu, HX
Xu, MQ
Li, SP
Tian, S
Guo, MX
Qi, JY
He, CJ
Zhao, XS
author_sort Liu, HX
collection PubMed
description Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the normal hepatocytes. JLGTE activated AMP-activated protein kinase (AMPK) signaling, which was required for its cytotoxicity against hepatocellular carcinoma cells. Silence of AMPKα1, via targeted short hairpin RNAs or CRISPR-Cas9 genome editing, inhibited JLGTE-induced AMPK activation and HepG2 cell apoptosis. Further, in-activation of AMPK by a dominant negative AMPKα1 (T172A) also alleviated JLGTE's cytotoxicity against HepG2 cells. On the other hand, forced-activation of AMPK by introduction of a constitutively-active AMPKα1 (T172D) mimicked JLGTE's actions and led to HepG2 cell apoptosis. These results suggest that JLGTE inhibits human hepatocellular carcinoma cells possibly via activating AMPK.
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spelling pubmed-57464042018-01-03 Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK Liu, HX Xu, MQ Li, SP Tian, S Guo, MX Qi, JY He, CJ Zhao, XS Oncotarget Research Paper Here we evaluated the anti-hepatocellular carcinoma activity by the Jujube leaf green tea extracts (JLGTE). We showed that JLGTE exerted anti-proliferative, cytotoxic and pro-apoptotic activities against HepG2 and primary human hepatocellular carcinoma cells. It was however non-cytotoxic to the normal hepatocytes. JLGTE activated AMP-activated protein kinase (AMPK) signaling, which was required for its cytotoxicity against hepatocellular carcinoma cells. Silence of AMPKα1, via targeted short hairpin RNAs or CRISPR-Cas9 genome editing, inhibited JLGTE-induced AMPK activation and HepG2 cell apoptosis. Further, in-activation of AMPK by a dominant negative AMPKα1 (T172A) also alleviated JLGTE's cytotoxicity against HepG2 cells. On the other hand, forced-activation of AMPK by introduction of a constitutively-active AMPKα1 (T172D) mimicked JLGTE's actions and led to HepG2 cell apoptosis. These results suggest that JLGTE inhibits human hepatocellular carcinoma cells possibly via activating AMPK. Impact Journals LLC 2017-11-30 /pmc/articles/PMC5746404/ /pubmed/29299169 http://dx.doi.org/10.18632/oncotarget.22821 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Liu, HX
Xu, MQ
Li, SP
Tian, S
Guo, MX
Qi, JY
He, CJ
Zhao, XS
Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title_full Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title_fullStr Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title_full_unstemmed Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title_short Jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating AMPK
title_sort jujube leaf green tea extracts inhibits hepatocellular carcinoma cells by activating ampk
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746404/
https://www.ncbi.nlm.nih.gov/pubmed/29299169
http://dx.doi.org/10.18632/oncotarget.22821
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