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Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform

The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low mole...

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Detalles Bibliográficos
Autores principales: Koukouvinos, Georgios, Petrou, Panagiota, Goustouridis, Dimitrios, Misiakos, Konstantinos, Kakabakos, Sotirios, Raptis, Ioannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746769/
https://www.ncbi.nlm.nih.gov/pubmed/29027976
http://dx.doi.org/10.3390/bios7040046
Descripción
Sumario:The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low molecular weight analytes (e.g., pesticides), is described. At the same time, the passage from single to multi-analyte determinations, and from a laboratory prototype set-up to a compact device appropriate for on-site determination, is outlined. The improvements made on both the sensor and the optical set-up, and the concomitant advances in the analytical characteristics and the robustness of the assays performed with the different layouts, are also presented. Finally, the future perspectives of the system, aiming for the creation of a standalone instrument to be used by non-experts, will be discussed.