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An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on t...

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Autores principales: Costantini, Francesca, Tiggelaar, Roald M., Salvio, Riccardo, Nardecchia, Marco, Schlautmann, Stefan, Manetti, Cesare, Gardeniers, Han J. G. E., de Cesare, Giampiero, Caputo, Domenico, Nascetti, Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746781/
https://www.ncbi.nlm.nih.gov/pubmed/29206205
http://dx.doi.org/10.3390/bios7040058
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author Costantini, Francesca
Tiggelaar, Roald M.
Salvio, Riccardo
Nardecchia, Marco
Schlautmann, Stefan
Manetti, Cesare
Gardeniers, Han J. G. E.
de Cesare, Giampiero
Caputo, Domenico
Nascetti, Augusto
author_facet Costantini, Francesca
Tiggelaar, Roald M.
Salvio, Riccardo
Nardecchia, Marco
Schlautmann, Stefan
Manetti, Cesare
Gardeniers, Han J. G. E.
de Cesare, Giampiero
Caputo, Domenico
Nascetti, Augusto
author_sort Costantini, Francesca
collection PubMed
description A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.
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spelling pubmed-57467812018-01-03 An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay Costantini, Francesca Tiggelaar, Roald M. Salvio, Riccardo Nardecchia, Marco Schlautmann, Stefan Manetti, Cesare Gardeniers, Han J. G. E. de Cesare, Giampiero Caputo, Domenico Nascetti, Augusto Biosensors (Basel) Article A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent. MDPI 2017-12-05 /pmc/articles/PMC5746781/ /pubmed/29206205 http://dx.doi.org/10.3390/bios7040058 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Costantini, Francesca
Tiggelaar, Roald M.
Salvio, Riccardo
Nardecchia, Marco
Schlautmann, Stefan
Manetti, Cesare
Gardeniers, Han J. G. E.
de Cesare, Giampiero
Caputo, Domenico
Nascetti, Augusto
An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_full An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_fullStr An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_full_unstemmed An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_short An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay
title_sort all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5746781/
https://www.ncbi.nlm.nih.gov/pubmed/29206205
http://dx.doi.org/10.3390/bios7040058
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