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Profiling the onset of somatic embryogenesis in Arabidopsis
BACKGROUND: Totipotency is the ability of a cell to regenerate a whole organism. Plant somatic embryogenesis (SE) is a remarkable example of totipotency because somatic cells reverse differentiation, respond to an appropriate stimulus and initiate embryo development. Although SE is an ideal system t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747089/ https://www.ncbi.nlm.nih.gov/pubmed/29284399 http://dx.doi.org/10.1186/s12864-017-4391-1 |
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author | Magnani, E. Jiménez-Gómez, J. M. Soubigou-Taconnat, L. Lepiniec, L. Fiume, E. |
author_facet | Magnani, E. Jiménez-Gómez, J. M. Soubigou-Taconnat, L. Lepiniec, L. Fiume, E. |
author_sort | Magnani, E. |
collection | PubMed |
description | BACKGROUND: Totipotency is the ability of a cell to regenerate a whole organism. Plant somatic embryogenesis (SE) is a remarkable example of totipotency because somatic cells reverse differentiation, respond to an appropriate stimulus and initiate embryo development. Although SE is an ideal system to investigate de-differentiation and differentiation, we still lack a deep molecular understanding of the phenomenon due to experimental restraints. RESULTS: We applied the INTACT method to specifically isolate the nuclei of those cells undergoing SE among the majority of non-embryogenic cells that make up a callus. We compared the transcriptome of embryogenic cells to the one of proliferating callus cells. Our analyses revealed that embryogenic cells are transcriptionally rather than metabolically active. Embryogenic cells shut off biochemical pathways involved in carbohydrate and lipid metabolism and activate the transcriptional machinery. Furthermore, we show how early in SE, ground tissue and leaf primordia specification are switched on before the specification of a shoot apical meristem. CONCLUSIONS: This is the first attempt to specifically profile embryogenic cells among the different cell types that constitute plant in vitro tissue cultures. Our comparative analyses provide insights in the gene networks regulating SE and open new research avenues in the field of plant regeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12864-017-4391-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5747089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57470892018-01-03 Profiling the onset of somatic embryogenesis in Arabidopsis Magnani, E. Jiménez-Gómez, J. M. Soubigou-Taconnat, L. Lepiniec, L. Fiume, E. BMC Genomics Research Article BACKGROUND: Totipotency is the ability of a cell to regenerate a whole organism. Plant somatic embryogenesis (SE) is a remarkable example of totipotency because somatic cells reverse differentiation, respond to an appropriate stimulus and initiate embryo development. Although SE is an ideal system to investigate de-differentiation and differentiation, we still lack a deep molecular understanding of the phenomenon due to experimental restraints. RESULTS: We applied the INTACT method to specifically isolate the nuclei of those cells undergoing SE among the majority of non-embryogenic cells that make up a callus. We compared the transcriptome of embryogenic cells to the one of proliferating callus cells. Our analyses revealed that embryogenic cells are transcriptionally rather than metabolically active. Embryogenic cells shut off biochemical pathways involved in carbohydrate and lipid metabolism and activate the transcriptional machinery. Furthermore, we show how early in SE, ground tissue and leaf primordia specification are switched on before the specification of a shoot apical meristem. CONCLUSIONS: This is the first attempt to specifically profile embryogenic cells among the different cell types that constitute plant in vitro tissue cultures. Our comparative analyses provide insights in the gene networks regulating SE and open new research avenues in the field of plant regeneration. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12864-017-4391-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-12-29 /pmc/articles/PMC5747089/ /pubmed/29284399 http://dx.doi.org/10.1186/s12864-017-4391-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Magnani, E. Jiménez-Gómez, J. M. Soubigou-Taconnat, L. Lepiniec, L. Fiume, E. Profiling the onset of somatic embryogenesis in Arabidopsis |
title | Profiling the onset of somatic embryogenesis in Arabidopsis |
title_full | Profiling the onset of somatic embryogenesis in Arabidopsis |
title_fullStr | Profiling the onset of somatic embryogenesis in Arabidopsis |
title_full_unstemmed | Profiling the onset of somatic embryogenesis in Arabidopsis |
title_short | Profiling the onset of somatic embryogenesis in Arabidopsis |
title_sort | profiling the onset of somatic embryogenesis in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747089/ https://www.ncbi.nlm.nih.gov/pubmed/29284399 http://dx.doi.org/10.1186/s12864-017-4391-1 |
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