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Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747455/ https://www.ncbi.nlm.nih.gov/pubmed/29287116 http://dx.doi.org/10.1371/journal.pone.0190445 |
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author | Dravet-Gounot, Pauline Morin, Cécile Jacques, Sébastien Dumont, Florent Ely-Marius, Fabiola Vaiman, Daniel Jarreau, Pierre-Henri Méhats, Céline Zana-Taïeb, Elodie |
author_facet | Dravet-Gounot, Pauline Morin, Cécile Jacques, Sébastien Dumont, Florent Ely-Marius, Fabiola Vaiman, Daniel Jarreau, Pierre-Henri Méhats, Céline Zana-Taïeb, Elodie |
author_sort | Dravet-Gounot, Pauline |
collection | PubMed |
description | Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We conducted a genome-wide analysis of gene expression and found the involvement of several pathways such as cell adhesion. Here, we describe our unbiased microRNA (miRNA) profiling by microarray assay and validation by qPCR at postnatal days 10 and 21 (P10 and P21) in lungs of rat pups with LPD-induced lung-alveolarization disorder after IUGR. We identified 13 miRNAs with more than two-fold differential expression between control lungs and LPD-induced IUGR lungs. Validated and predicted target genes of these miRNAs were related to “tissue repair” at P10 and “cellular communication regulation” at P21. We predicted the deregulation of several genes associated with these pathways. Especially, E2F3, a transcription factor involved in cell cycle control, was expressed in developing alveoli, and its mRNA and protein levels were significantly increased at P21 after IUGR. Hence, IUGR affects the expression of selected miRNAs during lung alveolarization. These results provide a basis for deciphering the mechanistic contributions of IUGR to impaired alveolarization. |
format | Online Article Text |
id | pubmed-5747455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57474552018-01-26 Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction Dravet-Gounot, Pauline Morin, Cécile Jacques, Sébastien Dumont, Florent Ely-Marius, Fabiola Vaiman, Daniel Jarreau, Pierre-Henri Méhats, Céline Zana-Taïeb, Elodie PLoS One Research Article Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We conducted a genome-wide analysis of gene expression and found the involvement of several pathways such as cell adhesion. Here, we describe our unbiased microRNA (miRNA) profiling by microarray assay and validation by qPCR at postnatal days 10 and 21 (P10 and P21) in lungs of rat pups with LPD-induced lung-alveolarization disorder after IUGR. We identified 13 miRNAs with more than two-fold differential expression between control lungs and LPD-induced IUGR lungs. Validated and predicted target genes of these miRNAs were related to “tissue repair” at P10 and “cellular communication regulation” at P21. We predicted the deregulation of several genes associated with these pathways. Especially, E2F3, a transcription factor involved in cell cycle control, was expressed in developing alveoli, and its mRNA and protein levels were significantly increased at P21 after IUGR. Hence, IUGR affects the expression of selected miRNAs during lung alveolarization. These results provide a basis for deciphering the mechanistic contributions of IUGR to impaired alveolarization. Public Library of Science 2017-12-29 /pmc/articles/PMC5747455/ /pubmed/29287116 http://dx.doi.org/10.1371/journal.pone.0190445 Text en © 2017 Dravet-Gounot et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Dravet-Gounot, Pauline Morin, Cécile Jacques, Sébastien Dumont, Florent Ely-Marius, Fabiola Vaiman, Daniel Jarreau, Pierre-Henri Méhats, Céline Zana-Taïeb, Elodie Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title | Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title_full | Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title_fullStr | Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title_full_unstemmed | Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title_short | Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
title_sort | lung microrna deregulation associated with impaired alveolarization in rats after intrauterine growth restriction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747455/ https://www.ncbi.nlm.nih.gov/pubmed/29287116 http://dx.doi.org/10.1371/journal.pone.0190445 |
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