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Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction

Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We...

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Autores principales: Dravet-Gounot, Pauline, Morin, Cécile, Jacques, Sébastien, Dumont, Florent, Ely-Marius, Fabiola, Vaiman, Daniel, Jarreau, Pierre-Henri, Méhats, Céline, Zana-Taïeb, Elodie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747455/
https://www.ncbi.nlm.nih.gov/pubmed/29287116
http://dx.doi.org/10.1371/journal.pone.0190445
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author Dravet-Gounot, Pauline
Morin, Cécile
Jacques, Sébastien
Dumont, Florent
Ely-Marius, Fabiola
Vaiman, Daniel
Jarreau, Pierre-Henri
Méhats, Céline
Zana-Taïeb, Elodie
author_facet Dravet-Gounot, Pauline
Morin, Cécile
Jacques, Sébastien
Dumont, Florent
Ely-Marius, Fabiola
Vaiman, Daniel
Jarreau, Pierre-Henri
Méhats, Céline
Zana-Taïeb, Elodie
author_sort Dravet-Gounot, Pauline
collection PubMed
description Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We conducted a genome-wide analysis of gene expression and found the involvement of several pathways such as cell adhesion. Here, we describe our unbiased microRNA (miRNA) profiling by microarray assay and validation by qPCR at postnatal days 10 and 21 (P10 and P21) in lungs of rat pups with LPD-induced lung-alveolarization disorder after IUGR. We identified 13 miRNAs with more than two-fold differential expression between control lungs and LPD-induced IUGR lungs. Validated and predicted target genes of these miRNAs were related to “tissue repair” at P10 and “cellular communication regulation” at P21. We predicted the deregulation of several genes associated with these pathways. Especially, E2F3, a transcription factor involved in cell cycle control, was expressed in developing alveoli, and its mRNA and protein levels were significantly increased at P21 after IUGR. Hence, IUGR affects the expression of selected miRNAs during lung alveolarization. These results provide a basis for deciphering the mechanistic contributions of IUGR to impaired alveolarization.
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spelling pubmed-57474552018-01-26 Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction Dravet-Gounot, Pauline Morin, Cécile Jacques, Sébastien Dumont, Florent Ely-Marius, Fabiola Vaiman, Daniel Jarreau, Pierre-Henri Méhats, Céline Zana-Taïeb, Elodie PLoS One Research Article Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We conducted a genome-wide analysis of gene expression and found the involvement of several pathways such as cell adhesion. Here, we describe our unbiased microRNA (miRNA) profiling by microarray assay and validation by qPCR at postnatal days 10 and 21 (P10 and P21) in lungs of rat pups with LPD-induced lung-alveolarization disorder after IUGR. We identified 13 miRNAs with more than two-fold differential expression between control lungs and LPD-induced IUGR lungs. Validated and predicted target genes of these miRNAs were related to “tissue repair” at P10 and “cellular communication regulation” at P21. We predicted the deregulation of several genes associated with these pathways. Especially, E2F3, a transcription factor involved in cell cycle control, was expressed in developing alveoli, and its mRNA and protein levels were significantly increased at P21 after IUGR. Hence, IUGR affects the expression of selected miRNAs during lung alveolarization. These results provide a basis for deciphering the mechanistic contributions of IUGR to impaired alveolarization. Public Library of Science 2017-12-29 /pmc/articles/PMC5747455/ /pubmed/29287116 http://dx.doi.org/10.1371/journal.pone.0190445 Text en © 2017 Dravet-Gounot et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dravet-Gounot, Pauline
Morin, Cécile
Jacques, Sébastien
Dumont, Florent
Ely-Marius, Fabiola
Vaiman, Daniel
Jarreau, Pierre-Henri
Méhats, Céline
Zana-Taïeb, Elodie
Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title_full Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title_fullStr Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title_full_unstemmed Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title_short Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
title_sort lung microrna deregulation associated with impaired alveolarization in rats after intrauterine growth restriction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5747455/
https://www.ncbi.nlm.nih.gov/pubmed/29287116
http://dx.doi.org/10.1371/journal.pone.0190445
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